文献类型： 外文期刊
作者： Yu, Qing 1 ; Li, Shuai 5 ; Su, Xiaofeng 1 ; Chen, Xinxiu 1 ; Dong, Yuanhua 1 ; Yao, Zhiwang 1 ; Jiang, Naiyu 1 ; Chai, Sen 1 ; Zhang, Zhonghua 1 ; Xu, Kuipeng 1 ;
作者机构： 1.Qingdao Agr Univ, Coll Hort, Engn Lab Genet Improvement Hort Crops Shandong Pro, Qingdao 266109, Shandong, Peoples R China
2.Qingdao Agr Univ, Minist Agr Rural Affairs, Key Lab Special Food Proc Coconstruct Minist & Pro, Qingdao 266109, Shandong, Peoples R China
3.Shandong Technol Innovat Ctr Special Food, Qingdao 266109, Shandong, Peoples R China
4.Qingdao Special Food Res Inst, Qingdao 266109, Shandong, Peoples R China
5.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Genome Anal Lab, Minist Agr, Shenzhen 518124, Guangdong, Peoples R China
6.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Hort Crops, Sino Dutch Joint Lab Hort Genom,Minist Agr, Beijing 100081, Peoples R China
关键词： Melon; Cucumis melo L.; Melon2K; Liquid SNP chip; Cultivar identification; Background selection
期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：6.2； 五年影响因子：6.1 ）
ISSN： 2095-9885
年卷期： 2025 年 11 卷 1 期
页码：
收录情况： SCI
摘要： High-throughput genotyping tools can effectively promote molecular breeding in crops. In this study, genotyping by target sequencing (GBTS) system was utilized to develop a genome-wide liquid SNP chip for facilitating genetics and breeding in melon (Cucumis melo L.), a globally cultivated economically important horticultural crop. Based on over eight million SNPs derived from 823 representative melon accessions, 16K, 8K, 4K, 2K, 1K, 500, 250 and 125 informative SNPs were screened and evaluated for their polymorphisms, conservation of flanking sequences, and distributions. The set of 2K SNPs was found to be optimal for representing the maximum diversity with the lowest number of SNPs, and it was selected to develop the liquid chip, named "Melon2K". Using Melon2K, more than 1500 SNPs were detected across 17 samples of five melon cultivars, and the phylogenetic relationships were clearly constructed. Within the same cultivar, genetic differences were also assessed between different samples. We evaluated the performance of Melon2K in genetic background selection during the breeding process, obtaining the introgression lines of interested trait with more than 97% genetic background of elite variety by only two rounds of backcrossing. These results suggest that Melon2K provides a cost-effective, efficient and reliable platform for genetic analysis and molecular breeding in melon.
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