Enhancing genetic transformation efficiency in cucurbit crops through AtGRF5 overexpression: Mechanistic insights and applications
文献类型： 外文期刊
作者： Li, Yang 1 ; Wang, Naonao 1 ; Feng, Jing 1 ; Liu, Yue 1 ; Wang, Huihui 1 ; Deng, Shijun 1 ; Dong, Wenjing 1 ; Liu, Xiaofeng 1 ; Lv, Bingsheng 1 ; Sun, Jinjing 2 ; Xu, Kuipeng 1 ; Zhang, Huimin 1 ; Zhang, Zhonghua 1 ; Chai, Sen 1 ;
作者机构： 1.Qingdao Agr Univ, Coll Hort, Engn Lab Genet Improvement Hort Crops Shandong Pro, Qingdao 266109, Peoples R China
2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China
关键词： auxin; cucurbit crops; genetic transformation; GRF; regeneration; scRNA-Seq
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.3； 五年影响因子：10.8 ）
ISSN： 1672-9072
年卷期： 2025 年 67 卷 7 期
页码：
收录情况： SCI
摘要： Transgenic and gene-editing technologies are essential for gene functional analysis and crop improvement. However, the pleiotropic effects and unknown mechanisms of morphogenic genes have hindered their broader application. In this study, we employed the one-step de novo shoot organogenesis (DNSO) method, and demonstrated that overexpression of the morphogenic gene Arabidopsis thanalia GROWTH-REGULATING FACTOR 5 (AtGRF5) significantly enhanced genetic transformation efficiency in cucurbit crops by promoting callus proliferation and increasing dense cells during regeneration. High-resolution time-series transcriptomics and single-cell RNA sequencing revealed that AtGRF5 overexpression induced auxin-related genes and expanded stem cell populations during cucumber DNSO. Using DNA-affinity purification sequencing (DAP-seq) in combination with spatiotemporal differential gene expression analysis, we identified CsIAA19 as a key downstream target of AtGRF5, with its modulation playing a pivotal role in regeneration. Rescuing CsIAA19 in AtGRF5-overexpressing explant reversed the enhanced callus proliferation and regeneration. To address growth defects caused by AtGRF5 overexpression, we developed an abscisic acid-inducible AtGRF5 expression system, significantly improving transformation and gene-editing efficiency across diverse genotypes while minimizing pleiotropic effects. In summary, this research provides mechanistic insights into AtGRF5-mediated transformation and offers a practical solution to overcome challenges in cucurbit crop genetic modification.
- 相关文献
作者其他论文 更多>>
-
Engineering tobacco for efficient astaxanthin production using a linker-free monocistronic dual-protein expression system and interspecific hybridization method
作者：Fang, Ning;Du, Zaifeng;Liu, Xiaofeng;Tian, Tian;Wang, Wenjing;Du, Yongmei;Zhang, Zhongfeng;Zhang, Hongbo;Chai, Maofeng;Zhao, Shancen;Timko, Michael P.;Xue, Zheyong
关键词：Metabolic engineering; Astaxanthin; Monocistronic cassette; Protein splicing; Tobacco
-
Deciphering the flavor constituents, microbiota and physicochemical properties of Yancaigao, a traditional sour rice paste from Southwest China
作者：Gao, Jinxiao;Li, Yang;Zhang, Yue;Shan, Yimeng;Wang, Aixia;Zhang, Xiya;Wang, Fengzhong;Tong, Li-Tao;Gao, Jinxiao;Luo, Tingting;Tong, Li-Tao
关键词：Sour rice paste; Lactic fermentation; Organic acids; Volatiles; Bacterial community
-
Melon2K array: A versatile 2K liquid SNP chip for melon genetics and breeding
作者：Yu, Qing;Su, Xiaofeng;Chen, Xinxiu;Dong, Yuanhua;Yao, Zhiwang;Jiang, Naiyu;Chai, Sen;Zhang, Zhonghua;Xu, Kuipeng;Zhang, Zhonghua;Zhang, Zhonghua;Zhang, Zhonghua;Li, Shuai;Li, Shuai
关键词：Melon; Cucumis melo L.; Melon2K; Liquid SNP chip; Cultivar identification; Background selection
-
A comprehensive review of starch-based technology for encapsulation of flavor: From methods, materials, and release mechanism to applications
作者：Shan, Yimeng;Li, Jiaxin;Nie, Mengzi;Li, Dezhi;Zhang, Yue;Li, Yang;Wang, Lili;Liu, Liya;Wang, Fengzhong;Tong, Li-Tao;Tong, Li-Tao
关键词：Encapsulation techniques; Flavor; Starch-based materials; Inclusion complex; Release mechanisms; Industry applications
-
Numerical Simulation Analysis and Research on Drag Reduction and Stability Enhancement Effect of Head Swing Based on Biomimetic Flexible Variants
作者：Qi, Debo;Li, Yang;Yang, Baisheng;Wu, Zhengyang;Li, Bo;Niu, Shichao
关键词：head swing; drag reduction; biomimetic flexible variants; radial drag; numerical simulation
-
Genome-wide analysis of the laccase gene family in Arachis hypogaea and functional characterization of AhLAC63 involved in lignin biosynthesis and abiotic stress
作者：Yao, Ruonan;Liu, Yue;Ouyang, Lei;Yan, Liying;Chen, Yuning;Huai, Dongxin;Wang, Zhihui;Kang, Yanping;Wang, Qianqian;Jiang, Huifang;Lei, Yong;Liao, Boshou;Wang, Xin;Yao, Ruonan;Ouyang, Lei;He, Dongli
关键词：Peanut; Laccase; Lignin; Abiotic stress; Phenylpropanoid
-
The PAP Gene Family in Cotton: Impact of Genome-Wide Identification on Fiber Secondary Wall Synthesis
作者：Sun, Cong;Li, Weijie;Qi, Ruiqiang;Liu, Yangming;Wang, Xiaoyu;Gong, Juwu;Gong, Wankui;Pan, Jingtao;Li, Yang;Shi, Yuzhen;Yan, Haoliang;Shang, Haihong;Yuan, Youlu;Wang, Xiaoyu;Shang, Haihong
关键词：cotton; purple acid phosphatase (PAP); cotton fiber; expression analysis
