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Heme catabolism mediated by heme oxygenase in uninfected interstitial cells enables efficient symbiotic nitrogen fixation in Lotus japonicus nodules

文献类型： 外文期刊

作者： Zhou, Yu 1 ; Wang, Longlong 1 ; Rubio, Maria Carmen 2 ; Perez-Rontome, Carmen 2 ; Zhou, Yumiao 1 ; Qi, Yongmei 1 ; Tian, Tao 1 ; Zhang, Weiqing 1 ; Fan, Qiuling 1 ; Becana, Manuel 2 ; Duanmu, Deqiang 1 ;

作者机构： 1.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Natl Key Lab Agr Microbiol, Hubei Hongshan Lab, Wuhan 430070, Peoples R China

2.CSIC, Dept Biol Vegetal, Estn Expt Aula, Ave Montanana 1005, Zaragoza 50059, Spain

3.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr,Genome Anal Lab,M, Shenzhen 518000, Peoples R China

关键词： heme homeostasis; heme oxygenase; leghemoglobin; Lotus japonicus; nodule senescence; symbiotic nitrogen fixation

期刊名称：NEW PHYTOLOGIST （ 影响因子：9.4； 五年影响因子：10.5 ）

ISSN： 0028-646X

年卷期： 2023 年 239 卷 5 期

页码：

收录情况： SCI

摘要： Legume nodules produce large quantities of heme required for the synthesis of leghemoglobin (Lb) and other hemoproteins. Despite the crucial function of Lb in nitrogen fixation and the toxicity of free heme, the mechanisms of heme homeostasis remain elusive.Biochemical, cellular, and genetic approaches were used to study the role of heme oxygenases (HOs) in heme degradation in the model legume Lotus japonicus. Heme and biliverdin were quantified and localized, HOs were characterized, and knockout LORE1 and CRISPR/Cas9 mutants for LjHO1 were generated and phenotyped.We show that LjHO1, but not the LjHO2 isoform, is responsible for heme catabolism in nodules and identify biliverdin as the in vivo product of the enzyme in senescing green nodules. Spatiotemporal expression analysis revealed that LjHO1 expression and biliverdin production are restricted to the plastids of uninfected interstitial cells. The nodules of ho1 mutants showed decreased nitrogen fixation, and the development of brown, rather than green, nodules during senescence. Increased superoxide production was observed in ho1 nodules, underscoring the importance of LjHO1 in antioxidant defense.We conclude that LjHO1 plays an essential role in degradation of Lb heme, uncovering a novel function of nodule plastids and uninfected interstitial cells in nitrogen fixation.

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