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Plant glutamyl-tRNA reductases coordinate plant and rhizobial heme biosynthesis in nitrogen-fixing nodules

文献类型： 外文期刊

作者： Wang, Longlong 1 ; Tian, Tao 1 ; Deng, Yan 1 ; Ji, Jie 1 ; Liang, Jianjun 1 ; Guan, Yuhao 1 ; Li, Runhui 3 ; Huang, Xiahe 4 ; Wang, Yingchun 4 ; Ning, Guogui 3 ; Fan, Qiuling 5 ; Becana, Manuel 6 ; Duanmu, Deqiang 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Agr Microbiol, Hubei Hongshan Lab, Wuhan 430070, Peoples R China

2.Anhui Agr Univ, Sch Agron, Hefei 230036, Peoples R China

3.Huazhong Agr Univ, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Peoples R China

4.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

5.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China

6.CSIC, Dept Biol Vegetal, Estn Expt Aula, Zaragoza 50059, Spain

7.Chinese Acad Agr Sci, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab Minist Agr,Agr Genom Inst Shenzhen, Shenzhen 518000, Peoples R China

期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.1 ）

ISSN： 1040-4651

年卷期： 2025 年 37 卷 5 期

页码：

收录情况： SCI

摘要： Heme is biosynthesized in legume root nodules to meet the demand for leghemoglobins (Lbs) and other heme-binding proteins. However, the main source of nodule heme remains unknown. Both the plant host and rhizobia possess a complete heme biosynthetic pathway, differing slightly in the production of 5-aminolevulinic acid (ALA), a key regulatory step catalyzed by glutamyl-tRNA reductase (GluTR) in the plant and by HemA in the rhizobia. Transcriptomic analysis revealed that many plant heme biosynthetic genes, including GluTR2 but not GluTR1, are upregulated in nodules compared to roots, whereas expression of related rhizobial genes, including both HemA1 and HemA2, is generally inhibited under symbiotic conditions compared to free-living conditions. Knockout of Lotus japonicus GluTR2, but not of HemA1 and HemA2, led to a significant decrease (similar to 50%) in nodule heme content. The stable heterozygous mutant of GluTR1 or transient knockdown of GluTR1 exhibited a similar to 20% reduction in nodule heme content. Overexpression of Fluorescent in blue light (FLU), a feedback inhibitor of GluTR activity, caused a much greater reduction in nodule heme content (similar to 75%) and an increased level of apo-Lb and, in combination with the hemA1 hemA2 mutant, a drastic inhibition of nitrogenase activity (>90%). This study provides genetic evidence supporting a major role of plant GluTRs in coordinating heme biosynthesis between the two symbionts by supplying heme to assemble with cytoplasmic apo-Lbs and by providing ALA for heme synthesis in the bacteroids.

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