Characterization of Flexusin A, a Novel Circular Bacteriocin Produced by Marine Bacterium Bacillus flexus R29-2
文献类型： 外文期刊
作者： Qiao, Xiaoni 1 ; Sun, Xiaowen 1 ; Wang, Shuting 1 ; Zhai, Chen 3 ; Tang, Wei 1 ; Tang, Tao 1 ; Zhang, Jun 1 ; He, Zengguo 2 ;
作者机构： 1.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China
2.Qingdao Bioantai Biotechnol Co Ltd, Qingdao 266000, Peoples R China
3.Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, Beijing 100193, Peoples R China
4.Marine Biomed Res Inst Qingdao, Marine Microbial Engn Res & Dev Ctr, Qingdao 266071, Peoples R China
关键词：
期刊名称：MARINE DRUGS （ 影响因子：5.4； 五年影响因子：5.6 ）
ISSN：
年卷期： 2025 年 23 卷 3 期
页码：
收录情况： SCI
摘要： Circular bacteriocins are potent antimicrobials against pathogenic Gram-positives. In searching for marine bacteriocins, an antibacterial peptide (flexusin A) was purified from the fermentation broth of marine bacterium Bacillus flexus R29-2. Genome sequencing and gene annotation revealed the chromosome contained an unknown circular bacteriocin gene cluster. Approaches including shot-gun proteomics analysis, AntiSMASH and BAGEL4 predication as well as the comprehensive sequence alignment, were then conducted, respectively, to verify the correlation of flexusin A with the gene-encoded precursor peptide. The results confirmed that flexusin A was the mature circular bacteriocin of the predicated precursor peptide with six amino acids as leader peptide. Flexusin A was 6098.4 Da in size, with a net charge of +3 and PI of 9.60. It shared the typical saposin-like fold spatial conformation features as commonly found in other circular bacteriocins. Flexusin A was pH, thermal, and protease tolerant. It exhibited a narrow antimicrobial spectrum against Gram-positives, and it can strongly inhibit Staphylococcus aureus by causing cell destruction via membrane destabilization. Taken together, a novel circular bacteriocin flexusin A was identified in this work. The characterization of flexusin A has extended circular bacteriocins family to 26 members. This is also the first report on bacteriocin production by B. flexus.
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