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Glyphosate and spinetoram alter viral communities with different effects on antibiotic resistance genes in the bumblebee gut

文献类型： 外文期刊

作者： Tang, Qihe 1 ; Zhao, Yazhou 2 ; Li, Xijie 1 ; Zhang, Jun 1 ; Li, Jilian 2 ; Zhao, Chonghui 1 ; Pang, Yantao 1 ; Li, Wanli 2 ; Huang, Qi 3 ; Xiong, Jian 4 ; Qian, Kai 5 ; Liu, Zhenxing 2 ; Guo, Jun 1 ;

作者机构： 1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Peoples R China

2.Chinese Acad Agr Sci, Inst Apicultural Res, State Key Lab Resource Insects, Beijing 100093, Peoples R China

3.Kunming Matern & Child Care Hosp, Kunming 650000, Yunnan, Peoples R China

4.Yunnan Zhongfeng Technol Dev Co Ltd, Kunming 651701, Yunnan, Peoples R China

5.Kunming Univ Sci & Technol, Affiliated Hosp, Dept Thorac Surg, Kunming, Peoples R China

关键词： Antibiotic resistance genes; Glyphosate; Spinetoram; Bumblebee; Lytic phages

期刊名称：JOURNAL OF ENVIRONMENTAL MANAGEMENT （ 影响因子：8.4； 五年影响因子：8.6 ）

ISSN： 0301-4797

年卷期： 2025 年 374 卷

页码：

收录情况： SCI

摘要： Limited research investigating the impact of pesticides on antibiotic resistance genes (ARGs) and viral community in the gut of wild animals. In this study, we employed metagenomic to investigate the effects of glyphosate and spinetoram on the gut viral communities, ARGs, and their interactions in a key wild pollinator, bumblebees. The results showed that both 2.5 mg/L glyphosate and 2.5 mg/L spinetoram did not significantly alter the alpha-diversity of the ARGs (p > 0.05). However, spinetoram significantly enriched core ARG subtypes, such as Bado_rpoB_RIF, Bbif_ileS_MUP, and CRP, and total abundance of ARGs (p < 0.05). In contrast, glyphosate had no significant impact on ARG subtypes or total abundance (p > 0.05). The mantel test (R = 0.455, p = 0.020) and Procrustes analysis (M-2 = 0.095, p = 0.069) revealed a significant correlation between the bacterial community and ARGs. Although glyphosate and spinetoram had no significant effect on the relative abundance of mobile ARGs (p > 0.05), both significantly altered the alpha diversity (p < 0.05) and compositional structure (one-way PERMANOVA, p = 0.003) of the gut viral communities, with glyphosate increasing the abundance of lytic phages (p < 0.05). Notably, a phage and host relationship network constructed revealed no evidence of phage-mediated ARGs transduction, but five associations between lytic phages and antibiotic-resistant bacteria (ARB) were identified. Furthermore, glyphosate and spinetoram exposure significantly reduced the total relative abundance of these five lytic phages in the viral community (p < 0.001), indicating that phages primarily function in lysing ARBs. These findings suggest that glyphosate may inhibit the enrichment of ARGs by increasing the abundance of lytic phages, while spinetoram may promote the enrichment of total ARGs by affecting the bacterial community.

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