Magnetic molecularly imprinted specific solid-phase extraction for determination of dihydroquercetin from Larix griffithiana using HPLC
文献类型： 外文期刊
作者： Ma, Xingbin 1 ; Zhang, Xukun 2 ; Lin, Hongling 3 ; AM, Abd El-Aty 4 ; Rabah, Tsdan 7 ; Liu, Xiaoxi 1 ; Yu, Zhichao 1 ; Yo 1 ;
作者机构： 1.Guangdong Ocean Univ, Dept Vet Med, Zhanjiang 524088, Guangdong, Peoples R China
2.Queens Univ, Coll Pharm, Belfast, Antrim, North Ireland
3.Chinese Acad Trop Sci, Southern Subtrop Crop Res Inst, Zhanjiang Expt Stn, Zhanjiang 524013, Peoples R China
4.Qilu Univ Technol, State Key Lab Biobased Mat & Green Papermaking, Shandong Acad Sci, Coll Food Sci & Engn, Jinan, Peoples R China
5.Cairo Univ, Dept Pharmacol, Fac Vet Med, Giza, Egypt
6.Ataturk Univ, Dept Med Pharmacol, Med Fac, Erzurum, Turkey
7.Tibet Acad Agr & Anim Husb Sci, Inst Vet & Anim Husb, Lhasa, Peoples R China
8.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agri Prod, Beijing 100081, Peoples R China
关键词： dihydroquercetin; Larix griffithiana; magnetic separation; molecularly imprinted polymers; Tibetan medicine
期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）
ISSN： 1615-9306
年卷期：
页码：
收录情况： SCI
摘要： The naturally occurring quercetin flavonoid, dihydroquercetin, is widely distributed in plant tissues and plays a variety of biological activities. Herein, a magnetic molecularly imprinted solid-phase extraction was tailor made for selective determination of dihydroquercetin in Larix griffithiana using high-performance liquid chromatography. Amino-functionalized core-shell magnetic nanoparticles were prepared and characterized using scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, and infrared spectroscopy. The polymer had an average diameter of 250 +/- 2.56 nm and exhibited good stability and adsorption for template molecule, which is enriched by hydrogen bonding interaction. Multiple factors for extraction, including loading, washing, elution solvents, and extraction time, were optimized. The limit of detection was 1.23 mu g/g. The precision determined at various concentration of dihydroquercetin was less than 4% and the mean recovery was between 74.64 and 101.80%. It has therefore been shown that this protocol can be used as an alternative extraction to quantify dihydroquercetin in L. griffithiana and purify quercetin flavonoid from other complex matrices.
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