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Analysis of the relationship between starch molecular conformation and enzymatic hydrolysis efficiency

文献类型： 外文期刊

作者： Haixia, Zhong 1 ; Xijuan, Yang 2 ; Yongxin, She 3 ; Guochao, Gan 1 ; Qiao, Wen 1 ; Li, Chen 1 ; Zhiguang, Chen 1 ;

作者机构： 1.Xichang Univ, Coll Agr Sci, Panxi Crops Res & Utilizat Key Lab Sichuan Prov, Xichang 615000, Sichuan, Peoples R China

2.Qinghai Univ, Acad Agr & Forestry Sci, Qinghai Tibetan Plateau Key Lab Agr Prod Proc, Qinghai 810016, Peoples R China

3.CAAS, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China

关键词： alpha-Amylase; Resistant starch; Molecular conformation

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2024 年 271 卷

页码：

收录情况： SCI

摘要： Resistant starch (RS) is important in controlling diabetes. The primary objective of this study is to examine the impact of molecular conformation on the enzymatic hydrolysis efficiency of starch by alpha-amylase. And the interactions between starch molecules with different conformations and alpha-amylase were analysed by using molecule dynamics simulation and molecular docking. It was found, the natural conformational starch molecule was hydrolysed from the middle of the starch chain by alpha-amylase, producing polysaccharides. The bent PSconformational starch molecules with multiple O 2 -O 3 intramolecular hydrogen bonds produced by highpressure was hydrolysed from the head of the starch chain to produce glucose, which is not conducive to RS formation. The stretched H-conformation without intramolecular hydrogen bonds produced by heat treatment was not hydrolysed by alpha-amylase. However, it occupied the active groove and formed strong interactions with alpha-amylase, which prevented other starch molecules from binding to alpha-amylase, thus reducing hydrolysis efficiency. Moreover, the total interaction energies between the three starch molecules and alpha-amylase were approximately 78 kJ/mol. And several hydrogen bonds were formed between the starch molecules and alpha-amylase, which provides evidence for the continuous sliding hydrolysis hypothesis of alpha-amylase. Moreover, these results provide an important reference for elucidating the mechanism of RS formation.

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