文献类型： 外文期刊

作者： Lin, Hongling ¹ ; Ma, Xingbin ² ; Zhou, Hanlin ¹ ; Shang, Xiaofei ¹ ; Wang, Shengyi ¹ ; Wei, Long ⁴ ; Li, Bing ¹ ; Bai, Yubin ¹ ; Wang, Weiwei ¹ ; Qiu, Yanhua ¹ ; Zhang, Chao ¹ ; Wang, Qing ¹ ; Zhou, Xuzheng ¹ ; She, Yongxin ⁵ ; Yuan, Ligang ³ ; Zhang, Jiyu ¹ ; Abd El-Aty, A. M. ⁶ ;

作者机构： 1.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab Vet Pharmaceut Dev, Minist Agr, Lanzhou 730050, Gansu, Peoples R China

2.Chinese Acad Trop Sci, Zhanjiang Expt Stn, Zhanjiang 524013, Peoples R China

3.Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Guangdong, Peoples R China

4.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Gansu, Peoples R China

5.Guangdong Acad Forestry, Guangdong Prov Key Lab Silviculture Protect & Util, Guangdong Coastal Shelter belt Forest Ecosyst Natl, Guangzhou 510520, Peoples R China

6.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agriprod, Beijing 100081, Peoples R China

7.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt

8.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkiye

关键词： Flavonoids; Acanthaceae ebracteatus; Extraction; Molecularly imprinted polymer; Determination

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）

ISSN： 0026-265X

年卷期： 2024 年 207 卷

页码：

收录情况： SCI

摘要： (Objectives) A novel method was developed for extracting and determining flavonoids from marine mangrove medicinal plants. This approach utilizes surface molecular imprinting to increase selectivity. Graphene oxide doped with a SiO2-Ag2O dual core structure served as a scaffold for synthesizing multilayer surface-imprinted polymers. The surface of the SiO2-Ag2O@GO composite was modified through self-polymerization. (Methods) Acacetin molecularly imprinted polymers (AMIPs) were created via surface-initiated precipitation polymerization with acacetin as the template. These AMIPs were synthesized and characterized via ultraviolet (UV) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and transmission electron microscopy (TEM). Recycling experiments demonstrated the stability of the AMIPs. Analysis of the adsorption mechanism via the Langmuir and Freundlich models indicated that the Langmuir model was more suitable for predicting AMIPs. (Results) Scatchard plots showing two different binding sites of AMIPs for acacetin adsorption at various concentrations. The class selectivity of AMIPs for flavonoids was found in Acanthaceae ebracteatus. Compared with other analogs, competitive binding experiments revealed that the polymer had greater selectivity for acacetin. After AMIP solid-phase extraction (AMISPE), the linearity, LOD, LOQ, intraday and interday variabilities, recovery, and repeatability of AMIPs combined with HPLC-MS/MS were validated, confirming the method's selectivity, precision, accuracy, and reproducibility. The contents of acacetin, apigenin, kaempferol, luteolin, quercetin, and genistein in Acanthaceae ebracteatus were determined. (Conclusions) These results indicate that AMISPE coupled with HPLC-MS/MS is a new, simple, and practical method for extracting, purifying, and enriching flavonoids from complex samples, such as those of herbs and other natural marine plants.