Selection and validation of reference genes for normalization of gene expression in Floccularia luteovirens
文献类型： 外文期刊
作者： Ni, Yanqing 1 ; Zhang, Qin 2 ; Li, Wensheng 1 ; Cao, Luping 2 ; Feng, Rencai 2 ; Zhao, Zhiqiang 2 ; Zhao, Xu 1 ;
作者机构： 1.Chengdu Univ, Coll Food & Biol Engn, Chengdu 610106, Sichuan, Peoples R China
2.Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610299, Sichuan, Peoples R China
3.Chengdu Natl Agr Sci & Technol Ctr, Chengdu 610299, Sichuan, Peoples R China
4.Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Gansu, Peoples R China
关键词： Floccularia luteovirens; RT-qPCR; Abiotic stresses; Reference gene
期刊名称：FUNGAL BIOLOGY （ 影响因子：2.5； 五年影响因子：3.0 ）
ISSN： 1878-6146
年卷期： 2024 年 128 卷 1 期
页码：
收录情况： SCI
摘要： Floccularia luteovirens is one of the rare edible fungi with high nutritional value found on the Qinghai-Tibet Plateau. However, research at the molecular level on this species is currently constrained due to the lack of reliable reference genes for this species. Thirteen potential reference genes (ACT, GAPDH, EF-Tu, SAMDC, UBI, CLN1, beta-TUB, gamma-TUB, GTP, H3, UBC, UBC-E2, and GTPBP1) were chosen for the present study, and their expression under various abiotic conditions was investigated. Stability of gene expression was tested using GeNorm, NormFinder, BestKeeper, Delta-Ct, and RefFinder. The results showed that the most suitable reference genes for salt treatment were ACT and EF-Tu. Under drought stress, gamma-TUB and UBC-E2 would be suitable for normalization. Under oxidative stress, the reference genes H3 and GAPDH worked well. Under heat stress, the reference genes EF-Tu and gamma-TUB were suggested. Under extreme pH stress, UBC-E2 and H3 were appropriate reference genes. Under cadmium stress, the reference genes ACT and UBC-E2 functioned well. In different tissues, H3 and GTPBP1 were appropriate reference genes. The optimal internal reference genes when analyzing all samples were H3 and SAMDC. The expression level of HSP90 was studied to further validate the applicability of the genes identified in this study.
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