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Poly(I:C) and CpG improve the assembly of foot-and-mouth disease virus-like particles and immune response in mice

文献类型： 外文期刊

作者： Liu, Haiyun 1 ; Teng, Zhidong 1 ; Dong, Hu 1 ; Li, Jiajun 1 ; Abdullah, Sahibzada Waheed 1 ; Zhang, Yun 1 ; Wu, Jinen 1 ; Guo, Huichen 1 ; Sun, Shiqi 1 ;

作者机构： 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Natl Foot and Mouth Dis Reference Lab, Lanzhou 730046, Peoples R China

关键词： Foot-and-mouth disease virus; Virus-like particles; CpG; Assembly

期刊名称：VIROLOGY （ 影响因子：3.7； 五年影响因子：3.2 ）

ISSN： 0042-6822

年卷期： 2023 年 579 卷

页码：

收录情况： SCI

摘要： Virus-like particles (VLPs) are extremely potent, safe, and serviceable vaccine platforms. Good assembly effi-ciency of VLPs is the key to reducing vaccine production costs and eliciting a robust immune response. This study adopted CpG and Poly (I:C) as scaffolds to facilitate the assembly of foot-and-mouth disease virus (FMDV) VLPs in vitro. The VLPs and the co-assembly products were characterized by particle size, zeta potential, gel retardation measurement, nuclease digestion experiments, size-exclusion chromatography, transmission electron microscopy and circular dichroism analysis. Our results indicated the successful encapsulation of CpG and Poly (I:C) inside VLPs without any effect on shape or size. Vaccination in mice also elicited a robust immune response. This study demonstrated that CpG and Poly (I:C) improved the efficiency of FMDV VLPs assembly and enhanced immune response, further proposing a new idea for improving the efficiency of VLPs assembly and enriching the in vitro VLPs assembly strategies.

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