Develop an indirect ELISA utilizing gD protein to detect antibodies against bovine herpesvirus type 1
文献类型： 外文期刊
作者： Liu, Qiang 1 ; Liang, Ruijin 2 ; Niu, Xiaoxia 1 ; Jiang, Lingling 1 ; Zhang, Gang 1 ; Wang, Pu 1 ; Zhang, Sinong 1 ; Gao, Weifeng 1 ; Wang, Yujiong 1 ; Guo, Huichen 1 ; Li, Yong 1 ;
作者机构： 1.Ningxia Univ, Sch Life Sci, Key Lab Minist Educ Protect & Utilizat Special Bio, Yinchuan, Peoples R China
2.China Med Univ, Queens Univ Belfast Joint Inst, Shenyang, Peoples R China
3.Lanzhou Univ, Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Coll Vet Med,State Key Lab Vet Etiol Biol, Lanzhou, Peoples R China
关键词： bovine herpesvirus type 1; gD protein; prokaryotic expression; indirect ELISA; antibody detection
期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：4.8； 五年影响因子：5.5 ）
ISSN： 2235-2988
年卷期： 2025 年 15 卷
页码：
收录情况： SCI
摘要： Bovine herpesvirus type 1 (BHV-1) is a highly contagious DNA virus that causes a variety of diseases affecting the reproductive and respiratory tracts. These diseases can reduce the health and production performance of cattle, causing significant economic losses in the cattle industry. The current ELISA kits used to detect BHV-1 have long lead times and are expensive, and are not suitable for bulk testing on large farms. therefore, there is an urgent need to develop a rapid and cost-effective alternative to the BHV-1 test. In this study, recombinant gD protein was expressed by prokaryotic system, and then used as antigen to immunize New Zealand white rabbits to obtain polyclonal antibodies (pAb). An indirect enzyme-linked immunosorbent assay (iELISA) based on gD protein was established for the detection of BHV-1 antibodies in clinical samples. The optimal cutoff value was determined to be 0.6185 using 60 clinical serum samples. This method had no cross-reaction with other common bovine viruses. The developed iELISA method and commercially available kits were used to detect 60 bovine serum samples, with a concordance rate of 93.3%. In summary, we established a rapid and reliable iELISA method based on gD protein, which is suitable for epidemio-logical monitoring of BHV-1.
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