您好，欢迎访问云南省农业科学院 机构知识库！

Genome-Wide Characterization and Sequence Polymorphism Analyses of Glycine max Fibrillin (FBN) Revealed Its Role in Response to Drought Condition

文献类型： 外文期刊

作者： Zafer, Muhammad Zeshan 1 ; Tahir, Muhammad Hammad Nadeem 2 ; Khan, Zulqurnain 1 ; Sajjad, Muhammad 3 ; Gao, Xiangkuo 4 ; Bakhtavar, Muhammad Amir 2 ; Waheed, Ummara 2 ; Siddique, Maria 5 ; Geng, Zhide 4 ; Ur Rehman, Shoaib 1 ;

作者机构： 1.Muhammad Nawaz Shareef Univ Agr, Inst Plant Breeding & Biotechnol, SINO PAK Joint Res Lab, Multan 66000, Pakistan

2.Muhammad Nawaz Shareef Univ Agr, Inst Plant Breeding & Biotechnol, Multan 66000, Pakistan

3.COMSATS Univ, Dept Biosci, Islamabad CUI, Pk Rd, Islamabad 45550, Pakistan

4.Yunnan Acad Agr Sci, Inst Food Crops, Kunming 650204, Peoples R China

5.COMSATS Univ Islamabad, Dept Environm Sci, Abbottabad Campus, Abbottabad 22060, Pakistan

关键词： Glycine max; fibrillin (FBN); genome-wide identification; marker-assisted breeding; CAPS; drought

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 6 期

页码：

收录情况： SCI

摘要： The fibrillin (FBN) gene family is widely distributed in all photosynthetic organisms. Members of this gene family are involved in plant growth and development and their response to various biotic and abiotic stress factors. In this study, 16 members of FBN were identified in Glycine max and characterized by using different bioinformatics tools. Phylogenetic analysis classified FBN genes into seven groups. The presence of stress-related cis-elements in the upstream region of GmFBN highlighted their role in tolerance against abiotic stresses. To further decipher the function, physiochemical properties, conserved motifs, chromosomal localization, subcellular localization, and cis-acting regulatory elements were also analyzed. Gene expression analysis based on FPKM values revealed that GmFBNs greatly enhanced soybean drought tolerance and controlled the expression of several genes involved in drought response, except for GmFBN-4, GmFBN-5, GmFBN-6, GmFBN-7 and GmFBN-9. For high throughput genotyping, an SNP-based CAPS marker was also developed for the GmFBN-15 gene. The CAPS marker differentiated soybean genotypes based on the presence of either the GmFBN-15-G or GmFBN-15-A alleles in the CDS region. Association analysis showed that G. max accessions containing the GmFBN-15-A allele at the respective locus showed higher thousand seed weight compared to accessions containing the GmFBN-15-G allele. This research has provided the basic information to further decipher the function of FBN in soybean.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台