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Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens

文献类型： 外文期刊

作者： Tao Li-li 1 ; Yin Gui-xiang 1 ; Du Li-pu 1 ; Shi Zheng-yuan 1 ; She Mao-yun 1 ; Xu Hui-jun 1 ; Ye Xing-guo 2 ;

作者机构： 1.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Crop Genet & Breeding, Minist Agr,Inst Crop Sci, Beijing 100081, Peoples R China

2.Chinese Acad Agr Sci, Natl Key Facil Crop Gene

关键词： wheat;immature embryos;Agrobacterium tumefaciens;transformation;genotypes;plant regeneration

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2011 年 10 卷 3 期

页码：

收录情况： SCI

摘要： Wheat, one of the most important food crops, has been extensively studied with respects to plant regeneration and transformation employing the immature embryos as recipient tissues. However, the transformed tissues often become severely necrotic after co-cultivation with Agrobacterium, which is one of the major obstacles in gene delivery. In this study, wheat varieties CB037, Kenong 199, Xinchun 9, Lunxuan 987, and Shi 4185 showed desirable culture potential or high infection ability in Agrobacterium-mediated transformation. Similarly, optimal regeneration conditions were determined by testing their ability to inhibit the cell necrosis and cell death phenotype. Two auxins of 2,4-dichlorophenoxyacetic acid (2,4-D) and 3,6-dichloro-o-anisic acid (dicamba) were evaluated for highly significant effect on both callus and plantlet production, although they were genotype-dependent in wheat. Substitution of 2,4-D by dicamba enhanced the growth and regeneration ability of callus from the immature embryos of most genotypes tested. The callus growth state couldn't be modified by adding antioxidants such as ascorbic acid, cysteine, and silver nitrate or organic additives such as thiamine HCl and asparagine to the media. On the contrary, the best tissue statement and plant regeneration was achieved by employing the media containing the simplest MS (Murashige and Skoog) components and dicamba without organic components and vitamins. Thereby, our results are thought to inhibit wheat cell necrosis effectively and suggested to be used for more wheat genotypes.

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