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Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)

文献类型： 外文期刊

作者： Wang Xin-min 1 ; Ren Xian; Yin Gui-xiang 1 ; Wang Ke 1 ; Li Jia-rui; Du Li-pu 1 ; Xu Hui-jun 1 ; Ye Xing-guo 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Beijing 100081, Peoples R China

关键词： wheat;immature embryos;environmental temperature;preservation;plant regeneration

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2014 年 13 卷 4 期

页码：

收录情况： SCI

摘要： The immature embryos (IEs) of wheat are the most widely used tissues for in vitro culture and genetic transformation due to its high regeneration competency. However, this explant can only be maintained in 4 degrees C daily cooler for a short period time for its use in plant tissue culture or transformation experiments. This study aimed to investigate the effects of environmental temperature, cryopreservation storage temperature, and heat shock culture (HSC) temperature on the regeneration frequency of wheat IEs. Results indicated that environmental temperature significantly affected the induction of embryonic calli. The optimum total accumulated temperature (TAT) during the time of anthesis and sampling for regeneration of these tissues was around 280 degrees C for spring wheat type cv. CB037 and approximately 300 degrees C for winter wheat type cv. Kenong 199. Regeneration ability obviously declined when the highest environmental temperature was over 35 degrees C for 1 d or a high temperature between 30 and 33 degrees C lasted for 5 d during anthesis and sampling. This finding was verified by culturing the freshly isolated IEs under different temperatures from 29 to 37 degrees C in different controlled growth incubators for 5 d; the IEs almost completely lost regeneration ability when the temperature rose to 37 degrees C. Cryopreservation of -20 degrees C caused the wheat samples lost ability of producing callus or embryonic callus in a few days, and cryopreservation of -10 degrees C more than 10 d made the regeneration potential of the tissues dramatically declined. Comparatively, the temperature that best maintained high regeneration ability was -5 degrees C, at which the materials can be maintained for around 1 mon. In addition, the preservation of the immature samples at -5 or -10 degrees C inhibited the direct germination of the IEs, avoiding the embryo axis removing process. Our results are useful for ensuring that field collection and cryopreservation of the wheat IEs are done correctly to enable tissue culture and generic transformation.

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