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Variation of the OsGI intron and its phenotypic associations in Oryza rufipogon Griff. and Oryza sativa L.

文献类型: 外文期刊

作者: Dong, Y. 1 ; Chen, Z. 1 ; Pei, X. 1 ; Wang, F. 2 ; Yuan, Q. 3 ; Wu, H. 4 ; Jia, S. 1 ; Peng, Y. 4 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Biotechnol, Beijing 100193, Peoples R China

2.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou, Guangdong, Peoples R China

3.Hainan Univ, Coll Agr Sci, MOE Key Lab Trop Biol Resources, Haikou, Peoples R China

4.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China

关键词: Oryza rufipogon;Oryza sativa;OsGI;Phylogenetic network;Cold tolerance;Seed dormancy

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2013 年 12 卷 3 期

页码:

收录情况: SCI

摘要: We analyzed intron 9 of the OsGI gene in Oryza rufipogon and Oryza sativa in order to investigate evolutionary relationships in rice and the relationship between intron variation and phenotype. OsGI-9 was cloned in 38 O. rufipogon populations and in 139 O. sativa cultivars and the phylogeny was reconstructed. Seed cold tolerance and dormancy were quantified in O. sativa. Three OsGI-9 band types occurred in O. rufipogon: S-type (1.2 kb), F-type (0.9 kb), and FS-type (1.2 and 0.9 kb), whereas only the S-type and F-type occurred in O. sativa. The S-type contains two 255-bp repeats, the F-type contains one 255-bp repeat, and the FS-type contains both. All individuals could be divided into 5 groups in the organism's phylogenetic network: S-type O. rufipogon, F-type O. rufipogon, FS-type O. rufipogon, S-type O. sativa, and F-type O. sativa. F-type O. sativa are most closely related to F-type O. rufipogon and S-type O. sativa are most closely related to S-type O. rufipogon. Statistical analysis indicated that OsGI-9 type is significantly correlated with phenotype; most S-type O. sativa have strong seed dormancy and cold tolerance, and most F-type O. sativa have no seed dormancy and poor cold tolerance.

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