文献类型： 外文期刊
作者： Du, X. 1 ; Ding, M. 1 ; Wu, Q. 1 ; Li, Ch 1 ; Guo, H. 1 ; Liu, G. 1 ; Chen, Z. 1 ;
作者机构： 1.Chinese Acad Agr Sci, Shanghai Vet Res Inst, 518 Ziyue Rd, Shanghai 200241, Peoples R China
关键词： duck reovirus; expression; characterization; novel P18 protein
期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）
ISSN： 0001-723X
年卷期： 2020 年 64 卷 1 期
页码：
收录情况： SCI
摘要： Novel duck reovirus (NDRV), the prototype strain of avian orthoreoviruses, continues to circulate among ducks. Analysis of its genome suggested that a putative second open reading frame in the Si segment encodes a 162-amino acid nonstructural protein with size of 18 kDa, provisionally designated P18. This protein is different from the 17 kDa nonstructural protein encoded in the same open reading frame in other avian orthoreoviruses, which is designated P17 and consists of 146 amino acids. There is no corresponding protein in Muscovy duck reovirus. Antibodies raised to the purified recombinant protein reacted with viral P18 both in vitro and in vivo. In cells, P18 was located predominantly in the nucleus at 6-12 h post-infection, with negligible levels in the cytoplasm. However, the protein accumulated both in the nucleus and cytoplasm at 24 to 36 h post-infection. Immunohistochemistry indicated that P18 strongly accumulates in spleen tissues of infected ducklings. Collectively, the data provide the direct experimental evidence that P18 is expressed by novel duck reovirus both in vivo and in vitro.
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