Construction of a bacterial artificial chromosome library for Gossypium herbaceum var. africanum
文献类型: 外文期刊
作者: Gao HaiYan 1 ; Wang XingFen 1 ; Liu Fang 1 ; Peng RenHai 1 ; Zhang Yan 1 ; Cheng Hua 1 ; Ma ZhiYing 1 ; Wang KunBo 1 ;
作者机构: 1.State Key Laboratory of Cotton Biology/Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China; North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Key Laboratory for Crop Germplasm Resources of Hebei Province, Agricultural University of Hebei, Baoding 071001, China
2.North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Key Laboratory for Crop Germplasm Resources of Hebei Province, Agricultural University of Hebei, Baoding 071001, China
3.State Key Laboratory of Cotton Biology/Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China
4.State Key Laboratory of Cotton Biology/Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China;Anyang Institute of Technology, Anyang 455000, China
5.Anyang Institute of Technology, Anyang 455000, China
关键词: wild cotton;Gossypium herbaceum var. africanum;bacterial artificial chromosome library;SSR marker;Verticillium wilt resistance
期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )
ISSN: 1001-6538
年卷期: 2013 年 58 卷 26 期
页码:
收录情况: SCI
摘要: Gossypium herbaceum var. africanum is the only wild cotton species within the cultivated diploid G. herbaceum. As the A sub-genome donor of tetraploid cotton, it is characterized by its resistance to insects, diseases, and other adversities. We have constructed the first bacterial artificial chromosome library (BAC) for G. herbaceum var. africanum. With high quality and broad coverage, this library includes 75000 clones, with an average insert size of about 115 kb and fewer than 4% empty clones. Our library is approximately five-fold the size of the A-genome (1667 Mb) and it provides 99.3% probability for isolating genes of interest or their sequences. Using nine SSR markers that are located on five different chromosomes and linked with resistance to Verticillium wilt, seven of nine could amplify the 40 superpools and got 1-14 hits. Because of its moderate wide coverage and relative large insert size, this library will be an important genomic resource for classifying and analyzing the evolution of cotton species, as well as for isolating disease-resistance genes and control elements.
- 相关文献
作者其他论文 更多>>
-
Characterization of a bla(CTX-M-3), bla(KPC-2) and bla(TEM-1B) co-producing IncN plasmid in Escherichia coli of chicken origin
作者:Wang Wen-jing;Wang Yi-fu;Tu Jian;Wang Wen-jing;Wang Yi-fu;Jin Ya-jie;Song Wu-qiang;Lin Jia-meng;Zhang Yan;Tong Xin-ru;Li Tao;Tu Jian;Li Rui-chao
关键词:bla(CTX-M-3); bla(KPC-2); bla(TEM-1B); IncN; plasmid; Escherichia coli
-
Genome-wide characterization of the Rho family in cotton provides insights into fiber development
作者:He Man;Wang Xingfen;He Man;Liu Shang;Cheng Hailiang;Zuo Dongyun;Wang Qiaolian;Lv Limin;Zhang Youping;Song Guoli;Cheng Hailiang
关键词:Cotton fiber; Cell polarity; Rho family; Association analysis; WGCNA
-
Knockdown of 60S ribosomal protein L14-2 reveals their potential regulatory roles to enhance drought and salt tolerance in cotton
作者:Shiraku, Margaret Linyerera;Magwanga, Richard Odongo;Cai Xiaoyan;Kirungu, Joy Nyangasi;Xu Yanchao;Mehari, Teame Gereziher;Hou Yuqing;Wang Yuhong;Wang Kunbo;Zhou Zhongli;Liu Fang;Magwanga, Richard Odongo;Peng Renhai;Liu Fang
关键词:Abiotic stress; Cotton; Ribosomal protein large; Transcription factor; Virus-induced gene silencing
-
Genome wide identification and characterization of light-harvesting Chloro a/b binding (LHC) genes reveals their potential role in enhancing drought tolerance in Gossypium hirsutum
作者:Mehari, Teame Gereziher;Xu Yanchao;Magwanga, Richard Odongo;Umer, Muhammad Jawad;Kirungu, Joy Nyangasi;Cai Xiaoyan;Hou Yuqing;Wang Yuhong;Yu Shuxun;Wang Kunbo;Zhou Zhongli;Fang, Liu;Mehari, Teame Gereziher;Magwanga, Richard Odongo;Fang, Liu
关键词:Cotton; G. hirsutum; LHC genes; Gene expression; Drought tolerance
-
Genetic map construction and functional characterization of genes within the segregation distortion regions (SDRs) in the F-2:3 populations derived from wild cotton species of the D genome
作者:Kirungu, Joy Nyangasi;Magwanga, Richard Odongo;Shiraku, Margaret Linyerera;Lu Pu;Mehari, Teame Gereziher;Xu Yuanchao;Hou Yuqing;Cai Xiaoyan;Zhou Zhongli;Wang Kunbo;Liu Fang;Magwanga, Richard Odongo;Agong, Stephen Gaya;Zhou Yun;Liu Fang
关键词:Genetic map; Segregation distortion region; Cis-regulatory elements; Genes; miRNA
-
Identification of QTLs and candidate genes for physiological traits associated with drought tolerance in cotton
作者:Magwanga, Richard Odongo;Lu Pu;Kirungu, Joy Nyangasi;Cai Xiaoyan;Zhou Zhongli;Wang Kunbo;Liu Fang;Magwanga, Richard Odongo;Agong, Stephen Gaya
关键词:Cotton spp.; Quantitative trait loci; Genetic map; Drought tolerance; miRNAs; Gene ontology
-
Identification and characterization of cell cultures with various embryogenic/regenerative potential in cotton based on morphological, cytochemical, and cytogenetical assessment
作者:Guo Hui-hui;Wu Jian-fei;Chen Cui-xia;Chu Zhao-hui;Zeng Fan-chang;Wang Hong-mei;Zhao Yun-lei;Zhang Chao-jun;Jia Yin-hua;Liu Fang;Ning Tang-yuan
关键词:cotton somatic embryogenesis; cotton cell culture strains; cytochemical EB-AC double staining; cytogenetical ploidy stability; embryogenic/regenerative potential; morphology



