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Identification and characterization of cell cultures with various embryogenic/regenerative potential in cotton based on morphological, cytochemical, and cytogenetical assessment

文献类型： 外文期刊

作者： Guo Hui-hui 1 ; Wu Jian-fei 1 ; Chen Cui-xia 1 ; Wang Hong-mei 2 ; Zhao Yun-lei 2 ; Zhang Chao-jun 2 ; Jia Yin-hua 3 ; Liu Fang 3 ; Ning Tang-yuan 4 ; Chu Zhao-hui 1 ; Zeng Fan-chang 1 ;

作者机构： 1.Shandong Agr Univ, State Key Lab Crop Biol, Minist Sci & Technol, Coll Agron, Tai An 271018, Shandong, Peoples R China

2.Chinese Acad Agr Sci, Minist Sci & Technol, State Key Lab Cotton Biol, Cotton Res Inst, Anyang 455000, Peoples R China

3.Chinese Acad Agr Sci, Minist Sci & Technol, State Key Lab Cotton

4.Key Laboratory of Water Resources and Drought Resistant Germplasm Improvement, Ministry of Agriculture, Tai’an 271018, P.R.China

关键词： cotton somatic embryogenesis; cotton cell culture strains; cytochemical EB-AC double staining; cytogenetical ploidy stability; embryogenic/regenerative potential; morphology

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2019 年 18 卷 1 期

页码：

收录情况： SCI

摘要： Somatic embryogenesis (SE) plays a vital role in genetic transformation and massive propagation of important agronomical and economical crops. Here, we conducted a systematic assessment of the morphological, cytochemical, and cytogenetical characteristics of six culture strains with various embryogenic/regenerative potential during SE process in cotton. Results indicated that the six cell culture strains had stable ploidy levels, and did not reveal any relationship between the cytogenetic state and their morphogenetic potential. Moreover, the six culture strains were compared via double staining with Evans blue and Acetocarmine to efficiently distinguish embryogenic and non-embryogenic cells and determine the embryogenic nature of the calli. In addition, the kind of auxins added in medium affected not only growth property, color, size of cell clumps but also ploidy level and regeneration ability. By combining analysis of morphological, cytochemical, and cytogenetical characteristics of the cell cultures, we are able to obtain and maintain homogeneous cell population with high morphogenic and regeneration ability and establish efficient somatic embryogenesis and regeneration system from short-term cell cultures in upland cotton, which highlight the application of biotechnological approaches in crop breeding, and above all, to better understand totipotency of cells in higher plants.

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