Gene expression changes in chicken NLRC5 signal pathway associated with in vitro avian leukosis virus subgroup J infection
文献类型： 外文期刊
作者： Qiu, L. L. 1 ; Xu, L. 1 ; Guo, X. M. 1 ; Li, Z. T. 1 ; Wan, F. 1 ; Liu, X. P. 2 ; Chen, G. H. 1 ; Chang, G. B. 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China
2.Chinese Acad Agr Sci, Poultry Inst, Yangzhou, Jiangsu, Peoples R China
关键词： Avian leukosis virus;Chicken embryo fibroblast;NLRC5;Signal pathway;Real-time PCR
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2016 年 15 卷 1 期
页码：
收录情况： SCI
摘要： Nucleotide-binding oligomerization domain-like receptors (NLRs) play a key role in the innate immune response as pattern-recognition receptors. However, the role of NLRC5, which is a member of the NLR family, in NF-kappa B activation and MHC-I expression remains debatable. Infection with the J group avian leukosis virus (ALV-J) can result in immunosuppression and a subsequent increase in susceptibility to secondary infection. This results in huge economic losses to the poultry industry worldwide. Using quantitative real-time polymerase chain reaction (qRT-PCR), we investigated the mRNA expression levels of NLRC5 signal pathway-related genes in secondary chicken embryo fibroblasts 7 days after infection with ALV-J. The results indicated that, compared with the control groups, the expression levels of TLR7, MHC-I, and IL-18 increased significantly in the infected groups at 7 days post-infection (d.p.i.). The expression levels of NLRC5 and IL-6 were conspicuously downregulated at 7 d.p.i., but the expression levels of NF-kappa B, STAT1, and STAT3 were not significantly altered. These results suggest that NLRC5 and some genes involved in the NLRC5 pathway play a key role in antiviral immunity, typically the response to ALV-J infection. Moreover, MHC-I expression levels vary between different cell types.
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