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Catalytic boost in dual-active sites hierarchical hollow nanozymes: An enhanced robust and sensitive immunochromatographic assay for Aspergillus flavus

文献类型： 外文期刊

作者： Liang, Meijuan 1 ; Hu, Xiaofeng 1 ; Wang, Du 1 ; Tang, Xiaoqian 1 ; Zhang, Qi 1 ; Li, Peiwu 1 ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs, Wuhan 430062, Peoples R China

2.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Detect Mycotoxins, Minist Agr & Rural Affairs, Wuhan 430062, Peoples R China

3.Xianghu Lab, Hangzhou 311231, Peoples R China

4.Hubei Hongshan Lab, Wuhan 430070, Peoples R China

5.Hubei Univ, Inst Food Safety, Wuhan 430062, Peoples R China

6.Minist Agr & Rural Affairs, Qual Inspection & Test Ctr Oilseed Prod, Wuhan 430062, Peoples R China

7.Minist Agr & Rural Affairs, Lab Qual & Safety Risk Assessment Oilseed Prod, Wuhan 430062, Peoples R China

关键词： Dual sites; Nanozyme; Catalytic efficiency; Robustness; Colorimetric lateral flow immunoassay; Aspergillus flavus

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.5； 五年影响因子：10.1 ）

ISSN： 0956-5663

年卷期： 2025 年 277 卷

页码：

收录情况： SCI

摘要： The practical application of nanozyme-mediated Point-of-Care Testing (POCT) is always constrained by insufficient sensitivity, slow development time, and limited robustness. Here, we developed a dual-active site hierarchical hollow CuS@MIL-100(Fe) (CuS@MIL) nanozyme through an easy-to-operate process. The resultant CuS@MIL nanozyme exhibited a 5- and 3-fold increase in catalytic efficiency relative to CuS and MIL-100, while the color development time was shortened by more than 6-fold. Meanwhile, CuS@MIL retained 91.4% of its catalytic activity after six months of storage, indicating its robustness. Density functional theory (DFT) revealed that the improved catalytic activity of CuS@MIL nanozyme was associated with low energy barriers for center dot OH generation and efficient interfacial electron transfer at Cu-Fe dual-active sites. Leveraging their high and stable catalytic performance, a sensitive colorimetric lateral flow immunoassay (LFA) was applied for detection of Aspergillus flavus (A. flavus), achieving a 200- and 5.6-fold increase in sensitivity compared to conventional and nanozyme-based colorimetric methods. Attractively, the LFA effectively detected A. flavus in peanuts with results closely matching those of ELISA, demonstrating the potential for rapid, efficient, sensitive and robust colorimetric POCT.

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