Time-resolved fluorescence/visual dual-readout nanobiosensors for the detection of aflatoxin B1, benzo(α)pyrene and capsaicin in edible oils using a miniaturized paper analytical device
文献类型： 外文期刊
作者： Huang, Xiaorong 1 ; Zhang, Qi 2 ; Lu, Baiyi 1 ; Tang, Xiaoqian 2 ; Li, Peiwu 1 ;
作者机构： 1.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Zhejiang, Peoples R China
2.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China
3.Minist Agr & Rural Affairs, Lab Risk Assessment Oilseed Prod Wuhan, Wuhan, Peoples R China
4.Minist Agr & Rural Affairs, Qual Inspect & Test Ctr Oilseed Prod, Wuhan, Peoples R China
5.Hubei Univ, Food Safety Res Inst, Wuhan 430062, Peoples R China
6.Hubei Hongshan Lab, Wuhan 430070, Peoples R China
7.Xianghu Lab, Hangzhou 311231, Peoples R China
关键词： BaP monoclonal antibody; Immune biosensor; Lateral flow strip; Multiple small molecular hazard factors; Food safety
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 467 卷
页码：
收录情况： SCI
摘要： Edible oil safety impacts food safety and consumer health. The typical pollutants-aflatoxin B1 (AFB1) and benzo (alpha)pyrene (BaP), and kitchen waste oil-are significant hazards in edible oil consumption. Herein, we developed a dual-readout lateral flow immunoassay (tdLFIA) for the multi-quantitative detection of AFB1, BaP and capsaicin (CAP). A novel monoclonal antibody against BaP was developed with a sensitivity of 1.92 ng/mL. Subsequently, gold nanoparticles and Eu3+ labelled fluorescent nanospheres were synthesized as colorimetric and fluorescent sensors. A rapid synchronous pretreatment method based on immunomagnetic beads(IMAB) combined with a molecularly imprinted solid phase extraction (MISPE) was developed. The tdLFIA enabled a rapid response of 7 min for AFB1, BaP and CAP detection, with quantitative limits of detection of 0.003, 0.6, and 0.01 ng/mL, respectively. The proposed strategy indicated reliability and has been applied in real samples, providing useful products for evaluating edible oil quality and safety.
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