Development of a "Signal-On" Fluorescent Aptasensor for Highly Selective and Sensitive Detection of ZEN in Cereal Products Using Nitrogen-Doped Carbon Dots Based on the Inner Filter Effect
文献类型： 外文期刊
作者： Sun, Qi 1 ; Zhou, Yuting 1 ; Ma, Miaomiao 1 ; Zhang, Fuyan 1 ; Li, Shuang 1 ; Chen, Zhuoer 1 ; Fang, Yu 1 ; Le, Tao 1 ; Xing, Fuguo 2 ;
作者机构： 1.Chongqing Normal Univ, Coll Life Sci, Chongqing Key Lab Conservat & Utilizat Freshwater, 37 Chengzhong Rd, Chongqing 401331, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Agroprod Qual & Safety Control Storage & T, Beijing 100193, Peoples R China
3.Chinese Acad Agr Sci, Inst Food Sci & Technol, Beijing 100193, Peoples R China
关键词： zearalenone; nitrogen-doped carbon dots; fluorescent aptasensor; detection mechanism; cereal products
期刊名称：BIOSENSORS-BASEL （ 影响因子：4.9； 五年影响因子：5.2 ）
ISSN：
年卷期： 2024 年 14 卷 7 期
页码：
收录情况： SCI
摘要： This study aimed to develop a novel fluorescent aptasensor for the quantitative detection of zearalenone (ZEN), addressing the limitations of conventional detection techniques in terms of speed, sensitivity, and ease of use. Nitrogen-doped carbon dots (N-CDs) were synthesized via the hydrothermal method, resulting in spherical particles with a diameter of 3.25 nm. These N-CDs demonstrated high water solubility and emitted a bright blue light at 440 nm when excited at 355 nm. The fluorescence of N-CDs was quenched by dispersed gold nanoparticles (AuNPs) through the inner filter effect, while aggregated AuNPs induced by NaCl did not affect the fluorescence of N-CDs. The aptamer could protect AuNPs from NaCl-induced aggregation, but the presence of ZEN weakened this protective effect. Based on this principle, optimal conditions for ZEN detection included 57 mM NaCl, 12.5 nM aptamer concentration, incubation of AuNPs with NaCl for 15 min in Tris-EDTA(TE) buffer, and incubation of aptamer with ZEN and NaCl for 30 min. Under these optimized conditions, the "signal-on" fluorescent aptasensor for ZEN detection showed a linear range of 0.25 to 200 ng/mL with a low detection limit of 0.0875 ng/mL. Furthermore, the developed aptasensor exhibited excellent specificity and could rapidly detect ZEN in corn flour samples or corn oil, achieving satisfactory recovery rates ranging from 84.7% to 108.6%. Therefore, this study presents an economical, convenient, sensitive, and rapid method for accurately quantifying ZEN in cereal products.
- 相关文献
作者其他论文 更多>>
-
Recent advances in microbial whole-cell biosensors for detecting food contaminants: a review
作者：Guan, Yue;Zheng, Mumin;Ma, Junning;Xing, Fuguo;Guan, Yue;Diez, Maria Suarez;Saccenti, Edoardo
关键词：Microbial whole-cell biosensors; Mycotoxins; Food pathogens; Heavy metals; Pesticides; Antibiotics
-
New application of a dye-decolorizing peroxidase immobilized on magnetic nanoparticles for efficient simultaneous degradation of two mycotoxins
作者：Du, Xinling;Zhang, Han;Qiu, Yangyu;Ji, Fuchun;Nie, Zishen;Xu, Huidong;Li, Xiaoxuan;Wu, Shijia;Wang, Zhouping;Xia, Yu;Zheng, Mumin;Xing, Fuguo
关键词：Dye-decolorizing peroxidase; Magnetic nanoparticle; Enzyme immobilization; Mycotoxin; Degradation
-
A node-localized transporter TaSPDT is responsible for the distribution of phosphorus to grains in wheat
作者：Wang, Aiying;Duan, Yaoke;Li, Shuang;Jiao, Zhen;Sun, Hao;Wang, Aiying;Duan, Yaoke;Wang, Rong;Li, Shuang;Cui, Keqiao;Gao, Feijuan;He, Bochao;Jiao, Zhen;Sun, Hao;Wang, Aiying;Kong, Xiaoping;Jiao, Zhen;Sun, Hao
关键词：wheat; phosphate transporter; phosphorus use efficient; node; sulfate transporter; xylem unloading; phloem reloading
-
Metarhizium spp. encode an ochratoxin cluster and a high efficiency ochratoxin-degrading amidohydrolase revealed by genomic analysis
作者：Wang, Gang;Ma, Junning;Xing, Fuguo;Wu, Wenqing;Guo, Xu;Li, Erfeng;Keller, Nancy P.
关键词：Secondary metabolite; Mycotoxin; OTA; Detoxification; Ochratoxigenic fungi
-
Functional Characterization and Mechanism of Two Zearalenone-Degrading Enzymes from Bacillus velezensis B.26
作者：Wei, Zifan;Shi, Lingfang;Wei, Zifan;Zhang, Xinyue;Shi, Lingfang;Jin, Jing;Yang, Bolei;Xing, Fuguo
关键词：
zearalenone ;Bacillus velezensis ;Degradation enzyme ;Degradation products ;Enzyme characteristics -
Inhibitory activity studies of the detoxifying bacterial strain "Lactobacillus brevis 121A" against Penicillium verrucosum growth and Ochratoxin A yield
作者：Humza, Muhammad;Imade, Francis Nosakhare;Liu, Yang;Humza, Muhammad;Xing, Fuguo;Liu, Yang;Zafar Khan, Muhammad Umar;Anwar, Muhammad Naveed;Sholkamy, Essam Nageh;Basit, Abdul;Iqbal, Babar;Durrani, Rida Haroon;Ali, Sanawar;Imade, Francis Nosakhare;Abdel-Raouf, Neveen;Alsamhary, Khawla I.;Abdel-Raouf, Neveen;Alharbi, Reem M.;Humza, Muhammad
关键词：
Penicillium verrucosum ; Ochratoxin A;Lactobacillus brevis ; Inhibition; Grapes -
Uncovering flavor and quality differences in Shuhua 9 peanuts dried at 35 ± 1 °C and 65 ± 1 °C using GC-IMS, untargeted metabolomics, and TMT-based proteomics
作者：Xu, Yongju;Hou, Rui;Li, Shuang;Yue, Fuliang;Zhang, Xiaohong;Zhang, Xiaojun;Li, Ling;Zhong, Kai
关键词：Shuhua 9 peanuts; Volatile compounds; GC-IMS; Metabolomics; Proteomics
