New application of a dye-decolorizing peroxidase immobilized on magnetic nanoparticles for efficient simultaneous degradation of two mycotoxins
文献类型： 外文期刊
作者： Du, Xinling 1 ; Zheng, Mumin 2 ; Zhang, Han 1 ; Qiu, Yangyu 1 ; Ji, Fuchun 1 ; Nie, Zishen 1 ; Xu, Huidong 1 ; Li, Xiaoxuan 1 ; Wu, Shijia 1 ; Wang, Zhouping 1 ; Xing, Fuguo 2 ; Xia, Yu 1 ;
作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
2.Chinese Acad Agr Sci, Inst Food Sci & Technol, Beijing 100193, Peoples R China
关键词： Dye-decolorizing peroxidase; Magnetic nanoparticle; Enzyme immobilization; Mycotoxin; Degradation
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 463 卷
页码：
收录情况： SCI
摘要： Nowadays the enzymatic approaches are the most promising strategies for mycotoxins detoxification in food stuffs. Herein, the dye-decolorizing peroxidase RhDypB from Rhodococcus jostii was studied for its ability to degrade two mycotoxins in both free and the immobilized enzyme forms. This enzyme was recombinantly expressed and purified, while Fe3O4 nanoparticles were prepared and modified with chitosan as the immobilization carrier. The immobilized enzyme Fe3O4@CS@RhDypB demonstrated degradation rate of 85.61 % toward aflatoxin B-1, while it was firstly found to be able to degrade zearalenone with the rate of 86.52 %, at pH 4.0 on 30 degrees C. The degradation products were identified as aflatoxin Q(1) and 15-OH-ZEN respectively. After 5 cycles of reuse, Fe3O4@CS@RhDypB still exhibited degradation rates of 38.50 % and 49.76 % toward the mycotoxins, indicating its high reusability. Moreover, Fe3O4@CS@RhDypB exhibited excellent stability after 10 days of storage. This work identified potential applications of nanoparticle-immobilized enzyme for biodegradation of mycotoxins in food industry.
- 相关文献
作者其他论文 更多>>
-
Quantitative estimation of blueberry SSC using fractional order derivative coupled optimized spectral indices
作者：Tian, Anhong;Zhang, Han;Tian, Anhong;Fu, Chengbiao;Fu, Chengbiao;Cao, Zhiyong;Li, Denghua;Li, Denghua
关键词：Blueberry SSC; Vis-NIR spectroscopy; Optimized spectral indices; Fractional order derivative; BPNN
-
Recent advances in microbial whole-cell biosensors for detecting food contaminants: a review
作者：Guan, Yue;Zheng, Mumin;Ma, Junning;Xing, Fuguo;Guan, Yue;Diez, Maria Suarez;Saccenti, Edoardo
关键词：Microbial whole-cell biosensors; Mycotoxins; Food pathogens; Heavy metals; Pesticides; Antibiotics
-
Metarhizium spp. encode an ochratoxin cluster and a high efficiency ochratoxin-degrading amidohydrolase revealed by genomic analysis
作者：Wang, Gang;Ma, Junning;Xing, Fuguo;Wu, Wenqing;Guo, Xu;Li, Erfeng;Keller, Nancy P.
关键词：Secondary metabolite; Mycotoxin; OTA; Detoxification; Ochratoxigenic fungi
-
Functional Characterization and Mechanism of Two Zearalenone-Degrading Enzymes from Bacillus velezensis B.26
作者：Wei, Zifan;Shi, Lingfang;Wei, Zifan;Zhang, Xinyue;Shi, Lingfang;Jin, Jing;Yang, Bolei;Xing, Fuguo
关键词：
zearalenone ;Bacillus velezensis ;Degradation enzyme ;Degradation products ;Enzyme characteristics -
Inhibitory activity studies of the detoxifying bacterial strain "Lactobacillus brevis 121A" against Penicillium verrucosum growth and Ochratoxin A yield
作者：Humza, Muhammad;Imade, Francis Nosakhare;Liu, Yang;Humza, Muhammad;Xing, Fuguo;Liu, Yang;Zafar Khan, Muhammad Umar;Anwar, Muhammad Naveed;Sholkamy, Essam Nageh;Basit, Abdul;Iqbal, Babar;Durrani, Rida Haroon;Ali, Sanawar;Imade, Francis Nosakhare;Abdel-Raouf, Neveen;Alsamhary, Khawla I.;Abdel-Raouf, Neveen;Alharbi, Reem M.;Humza, Muhammad
关键词：
Penicillium verrucosum ; Ochratoxin A;Lactobacillus brevis ; Inhibition; Grapes -
Exploring Therapeutic Targets From Spreading Patterns Against Respiratory Syncytial Virus
作者：Zhang, Han;Zhang, Zhao;Yang, Yixuan;Pei, Jingjing;Zhang, Han;Zhang, Zhao;Yang, Yixuan;Pei, Jingjing
关键词：respiratory syncytial virus (RSV); spreading patterns; therapeutic targets; viral protein receptor
-
Advantages of Multiphase Microwave Drying (MMD) Technology in Shiitake Mushroom Processing: Dual Optimization of Efficiency and Quality
作者：Liu, Zhenbin;Luo, Jia;Zhang, Jiayi;Li, Hongbo;Hu, Liangbin;Wang, Dong;Mo, Haizhen;Liu, Zhenbin;Luo, Jia;Zhang, Jiayi;Li, Hongbo;Hu, Liangbin;Mo, Haizhen;Liu, Wenchao;Hengchao, E.;Sun, Zhenying;Weng, Rui;Xia, Yu
关键词：Multiphase microwave drying; Shiitake mushrooms; Flavor retention; Nutrient preservation; Food drying efficiency
