您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Indirect signal amplification strategy with a universal probe-based lateral flow immunoassay for the rapid quantitative detection of fumonisin B1

文献类型： 外文期刊

作者： Zha, Chuanyun 1 ; An, Xingshuang 1 ; Zhang, Jiali 1 ; Lin Wei 1 ; Zhang, Qi 4 ; Yang, Qingqing 1 ; Li, Falan 1 ; Sun, Xia 1 ; Guo, Yemin 1 ;

作者机构： 1.Shandong Univ Technol, Sch Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China

2.Shandong Prov Engn Res Ctr Vegetable Safety & Qua, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China

3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China

4.Chinese Acad Agr Sci, Oil Crops Res Inst, 2 Xudong 2nd Rd, Wuhan 430062, Peoples R China

期刊名称：ANALYTICAL METHODS （ 影响因子：3.532； 五年影响因子：3.244 ）

ISSN： 1759-9660

年卷期： 2022 年 14 卷 7 期

页码：

收录情况： SCI

摘要： Fumonisin B1 (FB1) is a serious threat to the health of humans and animals. Herein, a lateral flow immunoassay based on universal detection probes (goat anti-mouse IgG@Eu) that could combine with any mouse monoclonal antibody was applied to detect FB1 in corn and feed. Compared with that based on direct monoclonal antibody labeling, this assay maintained bioactivity and saved consumption of monoclonal antibodies with the indirect signal amplification effect. The results indicated that this assay had higher sensitivity with a limit of detection (LOD) of 0.025 and 0.097 ng mL(-1) (0.50 and 1.94 ng g(-1) based on sample weight) in corn and feed, respectively. The detection range was about 1-50 ng mL(-1) (20-1000 ng g(-1) based on sample weight). In addition, the evaluation proved that it had good specificity, accuracy, precision, and applicability, and thus was suitable for the rapid and low-cost detection of fumonisin B1.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台