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Generation of chicken induced pluripotent stem cells (iPSCs) from somatic cells using multi-lentiviral transduction and OAC2

文献类型： 外文期刊

作者： Liu, Guangzheng 1 ; Zhu, Xilin 1 ; Li, Zeyu 1 ; Zhu, Liqian 1 ; Chen, Guohong 1 ; Niu, Yingjie 1 ; Sun, Hongyan 1 ; Zuo, Qisheng 1 ; Song, Jiuzhou 4 ; Han, Wei 5 ; Wei, Wanhong 6 ; Li, Bichun 1 ; Jin, Kai 1 ;

作者机构： 1.Yangzhou Univ, Minist Educ China, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Peoples R China

2.Yangzhou Univ, Coll Anim Sci & Technol, Key Lab Anim Breeding Reprod & Mol Design Jiangsu, Yangzhou 225009, Peoples R China

3.Yangzhou Univ, Inst Agr Sci & Technol Dev, Yangzhou 225009, Peoples R China

4.Univ Maryland, Dept Anim & Avian Sci, College Pk, MD USA

5.Chinese Acad Agr Sci, Jiangsu Inst Poultry Sci, Poultry Inst, Yangzhou 225003, Peoples R China

6.Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China

7.Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang 212100, Peoples R China

关键词： Chicken; iPSCs; Somatic cell reprogramming; Multi-lentiviral; OAC2

期刊名称：THERIOGENOLOGY （ 影响因子：2.5； 五年影响因子：2.7 ）

ISSN： 0093-691X

年卷期： 2025 年 247 卷

页码：

收录情况： SCI

摘要： The chicken iPSCs have been successfully established and exhibit significant potential for the recovery and conservation of endangered avian species. However, the efficiency of induction remains quite limited. To enhance the induction system, a six-factor reprogramming approach incorporating OCT4, SOX2, NANOG, LIN28, KLF4, and C-MYC (OSNLKM) was employed via lentiviral infection, in combination with the small-molecule compound OAC2 and mouse embryonic fibroblast (MEF) feeder layers. The results demonstrated that the sixfactor system significantly accelerated the reprogramming process and improved induction efficiency compared to the conventional four-factor system (OCT4, SOX2, NANOG, and LIN28; OSNL). Additionally, the MEF feeder layers were found to be essential for the successful reprogramming of chicken somatic cells. The inclusion of Oct4-activating compound 2 (OAC2) further optimized the reprogramming conditions, enabling the successful induction of iPSCs from chicken embryonic fibroblasts (CEFs) and Sertoli cells. Collectively, this study establishes an optimized reprogramming system for chicken somatic cells, enhancing iPSCs induction efficiency and expanding the potential applications of iPSCs technology in avian conservation and genetic modification.

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