Functional Equivalence of Insulin and IGF-1 in the In Vitro Culture of Chicken Primordial Germ Cells
文献类型： 外文期刊
作者： Liu, Xin 1 ; Wu, Jun 1 ; Peng, Yixiu 1 ; Liu, Guangzheng 1 ; Jin, Kai 1 ; Niu, Yingjie 1 ; Song, Jiuzhou 3 ; Han, Wei 4 ; Chen, Guohong 1 ; Li, Bichun 1 ; Zuo, Qisheng 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Mol Design Jiangsu P, Yangzhou 225009, Peoples R China
2.Yangzhou Univ, Minist Educ China, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Peoples R China
3.Univ Maryland, Anim & Avian Sci, College Pk, MD 20742 USA
4.Chinese Acad Agr Sci, Poultry Inst, Poultry Inst Jiangsu, Yangzhou 225003, Peoples R China
5.Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang 212100, Peoples R China
关键词： chicken PGCs; IGF-1; migration; establishment efficiency
期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 16 卷 5 期
页码：
收录情况： SCI
摘要： Background: Chicken Primordial Germ Cells (PGCs) are one of the few germ cells that can be cultured for a long time in vitro, but challenges remain such as low culture efficiency and unclear roles of nutrient factors and signaling pathways. Method: In this study, protein kinase B (AKT) pathway activator insulin-like growth factor 1 (IGF-1) was screened for its ability to promote cell proliferation by transcriptome results using various inhibitors of pathway activation. The effects of IGF-1 on PGCs were evaluated through EdU assays, qRT-PCR, flow cytometry, and migration experiments. Results: This study systematically examined the effects of insulin and IGF-1 on the proliferation, cell cycle, ferroptosis, migration capacity, and establishment efficiency of PGCs. The findings demonstrated that IGF-1 exhibited comparable effects to insulin and could effectively replace insulin in PGC culture systems. Conclusions: The research results are expected to provide a solid theoretical basis for optimizing the chicken PGC cultivation system and promoting its practical application.
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