您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Improved fluorescence-based assay for rapid screening and evaluation of SARS-CoV-2 main protease inhibitors

文献类型： 外文期刊

作者： Zhang, Rui 1 ; Yan, Haohao 1 ; Zhou, Jiahao 1 ; Yan, Gangan 1 ; Liu, Xiaoping 1 ; Shang, Chao 2 ; Chen, Yunyu 1 ;

作者机构： 1.Wannan Med Coll, Inst Drug Screening & Evaluat, Wuhu 241002, Peoples R China

2.Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun 130122, Peoples R China

关键词： echinatin; high-throughput screening; main protease inhibitor; MG-101; red fluorescent protein; SARS-CoV-2

期刊名称：JOURNAL OF MEDICAL VIROLOGY （ 影响因子：6.8； 五年影响因子：6.6 ）

ISSN： 0146-6615

年卷期： 2024 年 96 卷 3 期

页码：

收录情况： SCI

摘要： The outbreak of coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global threat to human health. In parallel with vaccines, efficacious antivirals are urgently needed. SARS-CoV-2 main protease (Mpro) is an attractive drug target for antiviral development owing to its key roles in virus replication and host immune evasion. Due to the limitations of currently available methods, the development of novel high-throughput screening assays is of the highest importance for the discovery of Mpro inhibitors. In this study, we first developed an improved fluorescence-based assay for rapid screening of Mpro inhibitors from an anti-infection compound library using a versatile dimerization-dependent red fluorescent protein (ddRFP) biosensor. Utilizing this assay, we identified MG-101 as a competitive Mpro inhibitor in vitro. Moreover, our results revealed that ensitrelvir is a potent Mpro inhibitor, but baicalein, chloroquine, ebselen, echinatin, and silibinin are not. Therefore, this robust ddRFP assay provides a faithful avenue for rapid screening and evaluation of Mpro inhibitors to fight against COVID-19.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台