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Comprehensive analysis of the transcriptome-wide m6A Methylome in sheep testicular development

文献类型： 外文期刊

作者： Xi, Binpeng 1 ; Lu, Zengkui 1 ; Zhang, Rui 1 ; Zhao, Shengguo 3 ; Li, Jianye 1 ; An, Xuejiao 1 ; Yue, Yaojing 1 ;

作者机构： 1.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab Anim Genet & Breeding Tibetan Plateau, 335 Jiangouyan, Lanzhou 730050, Gansu, Peoples R China

2.Chinese Acad Agr Sci, Sheep Breeding Engn Technol Res Ctr, Lanzhou 730050, Peoples R China

3.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou 730070, Peoples R China

关键词： mRNA m6A methylation; Testicular development; F1 hybrid of Southdown x Hu sheep; Spermatogenesis

期刊名称：GENOMICS （ 影响因子：3.0； 五年影响因子：3.3 ）

ISSN： 0888-7543

年卷期： 2025 年 117 卷 2 期

页码：

收录情况： SCI

摘要： N6-methyladenosine (m6A) modification of RNA is a critical post-transcriptional modification, that dynamically contributes to testicular development and spermatogenesis. Nevertheless, the investigation into the role of m6A in testicular development of sheep remains insufficient. Herein, we conducted a comprehensive analysis of the m6A transcriptome landscape in the testes of F1 hybrid Southdown x Hu sheep across M0 (0 months old, newborn), M3 (3 months old, sexually immature), M6 (6 months old, sexually mature), and Y1 (1 years old, adult). By profiling the m6A signatures across the transcriptome, we observed distinct differences in m6A modification patterns during sheep testicular development. Our cross-analysis of m6A and mRNA expression revealed that the expression of 743 genes and their m6A modification were concurrent. Notably, the combined analysis of the two comparative groups, M0 vs. M6 and M0 vs. Y1, exhibited a positive correlation, with 30 candidate genes each located within the largest protein-protein interaction network. Intriguingly, eight key hub genes (VEGFA, HDAC9, ZBTB40, KDM5B, MTRR, EAPS1, TSSK3, and BMP4) were identified to be associated with the regulation of sheep testicular development and spermatogenesis. These findings contribute to a deeper understanding of the dynamic role of m6A modification in sheep testicular biology. This study to map RNA m6A modification in sheep testes at different ages, providing novel insights into m6A topology and the molecular mechanisms associated with spermatogenesis in Southdown x Hu sheep F1 hybrids and laying the foundation for further investigations of mammalian spermatogenesis.

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