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Peptide aptamer-based colorimetric sensor for the detection of L-tryptophan in porcine serum

文献类型： 外文期刊

作者： Wang, Wenjing 1 ; He, Yumin 1 ; He, Suxiang 1 ; Liu, Xiaoying 3 ; Gui, Qing-wen 3 ; Deng, Lei 4 ; Wang, Hui 1 ; Cao, Zhong 5 ; Feng, Zemeng 1 ; Xiong, Benhai 7 ; Yin, Yulong 1 ;

作者机构： 1.Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc,, Inst Subtrop Agr,Key Lab Agroecol Proc Subtrop Reg, Natl Engn Lab Pollut Control & Waste Utilizat Live, Changsha 410125, Peoples R China

2.Univ South China, Hengyang Med Sch, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China

3.Hunan Agr Univ, Coll Chem & Mat Sci, Changsha 410128, Hunan, Peoples R China

4.Cent South Univ, Sch Comp Sci & Engn, Changsha 410075, Hunan, Peoples R China

5.Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Trans, Changsha 410114, Hunan, Peoples R China

6.Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Cytochem, Changsha 410114, Hunan, Peoples R China

7.Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, Beijing 100193, Peoples R China

关键词： Peptide aptamer; Gold nanoparticle; L-tryptophan; Colorimetric sensor

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：4.8； 五年影响因子：4.5 ）

ISSN： 0026-265X

年卷期： 2024 年 197 卷

页码：

收录情况： SCI

摘要： L-Tryptophan (L-Trp), one of the essential amino acids, is crucial for human physiological homeostasis and a limiting amino acid in animal feed. In addition, L-Trp is a precursor of some important biomolecules in the body, such as pentraxin and melatonin. Disorders of L-Trp metabolism in the body may cause Alzheimer's or Parkinson's disease. Therefore, monitoring L-Trp levels in the body fluids quickly and accurately is essential. A colorimetric biosensor was developed for the rapid detection of L-Trp in solutions by coupling a novel, screened, and validated peptide aptamer with Au nanoparticles via the Au-S bond. The biosensor showed a wide linear detection range of 1 mu M - 1000 mu M, and in addition, it was simple to construct, and the reaction time was as short as 10 s. In the analysis of L-Trp in actual porcine serum samples, the relative standard deviations were 4.52 % to 3.01 % compared with those of the high-performance liquid chromatography method, and the spiked recoveries were 99.2 % to 100.2 %. This new type of recognition probe is likely to gain attention in the field of bioanalysis owing to its biocompatibility, ease of modification, and easy linkage to nanomaterials.

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