Liver Transcriptome Analysis Reveals a Potential Mechanism of Heat Stress Increasing Susceptibility to Salmonella Typhimurium in Chickens
文献类型： 外文期刊
作者： Zhang, Qi 1 ; Zhu, Yvqing 1 ; Wang, Zixuan 1 ; Li, Qinghe 1 ; Zhao, Guiping 1 ; Wang, Qiao 1 ;
作者机构： 1.Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, 2 Yuanmingyuan W Rd, Beijing 100193, Peoples R China
关键词：
chicken;
期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）
ISSN：
年卷期： 2025 年 14 卷 6 期
页码：
收录情况： SCI
摘要： Salmonella infection poses a serious threat to the poultry industry, causing significant economic losses. Under global warming conditions, the underlying molecular mechanisms by which heat stress affects bacterial infections in poultry remain unclear. This study conducted a Salmonella Typhimurium infection under heat stress in Guang Ming broilers. A total of 100 chickens were randomly divided into three groups: control group (CTL), Salmonella Typhimurium (ST) infection group, and heat stress and Salmonella Typhimurium (HS + ST) co-stimulation group. By integrating inflammatory phenotypes, liver transcriptome profiles, and weighted gene co-expression network analysis (WGCNA), we systematically investigated the key regulatory factors through which heat stress affects host susceptibility to Salmonella. The results demonstrated that heat stress reduced body weight gain, exacerbated Salmonella Typhimurium-induced inflammatory responses, and increased mortality. Transcriptome results revealed that heat stress led to excessive inflammatory responses and antioxidant defense imbalances. Combined differential expression analysis and WGCNA identified three hub regulatory genes: PTGDS and WISP2 showed significant correlations with the heterophil/lymphocyte ratio, while SLC6A9 was significantly correlated with serum IL-8 levels. Validation in HD11 cell infection models confirmed the differential expression of these genes under heat stress and Salmonella Typhimurium co-stimulation, indicating their critical roles in host immune regulation. This study elucidates the intrinsic regulatory relationships through which heat stress promotes Salmonella pathogenicity and inflammatory responses, providing important insights for disease-resistant poultry breeding and prevention strategies.
- 相关文献
作者其他论文 更多>>
-
Development of an enhanced hybrid attention YOLOv8s small object detection method for phenotypic analysis of root nodules
作者：Zhao, Ya;Zhang, Wen;Zhang, Liangxiao;Tang, Xiaoqian;Wang, Du;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Zhao, Ya;Li, Peiwu
关键词：Root nodules; YOLOv8s; Hybrid attention mechanism; Small object detection; Phenotypic characteristics
-
Smartphone-assisted electrochemiluminescence imaging test strips towards dual-signal visualized and sensitive monitoring of aflatoxin B1 in corn samples
作者：Chen, Miao-Miao;Jiang, Jun;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Chen, Miao-Miao;Jiang, Jun;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Chen, Miao-Miao;Zhang, Min-Ling;Song, Xiao;Zhang, Xiuhua;Chen, Miao-Miao;Song, Xiao
关键词：Electrochemiluminescence imaging; Test strips; Nanomaterials; Dual-signal outputs; Aflatoxin B1; Food safety
-
Dual-channel microfluidic paper-based fluorescent immunosensor for simultaneous detection of aflatoxin B1 and capsaicin in edible oils
作者：Girmatsion, Mogos;Tang, Xiaoqian;Zhang, Qi;Wu, Wenqin;Hu, Xiaofeng;Li, Peiwu;Girmatsion, Mogos;Tang, Xiaoqian;Zhang, Qi;Wu, Wenqin;Hu, Xiaofeng;Li, Peiwu;Girmatsion, Mogos;Tang, Xiaoqian;Zhang, Qi;Wu, Wenqin;Hu, Xiaofeng;Li, Peiwu;Girmatsion, Mogos;Tang, Xiaoqian;Zhang, Qi;Wu, Wenqin;Hu, Xiaofeng;Li, Peiwu;Girmatsion, Mogos;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Girmatsion, Mogos;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Girmatsion, Mogos;Zhang, Qi;Li, Peiwu;Girmatsion, Mogos
关键词：Dual-channel paper device; Immunosensor; Phycocyanin; Aflatoxin B 1; Capsaicin; Edible oils
-
Single-cell RNA sequencing of the spleen reveals differences in Salmonella typhimurium infection mechanisms between different chicken breeds
作者：Zhang, Qi;Wang, Qiao;Zheng, Jumei;Zhang, Jin;Zhang, Gaomeng;Wen, Jie;Li, Qinghe;Zhao, Guiping;Ying, Fan;Liu, Dawei
关键词：Chicken; Disease resistance; Single-cell RNA sequencing; Bacterial infection; CD45+cells
-
Carposphere microbiota alters grape volatiles and shapes the wine grape typicality
作者：Liu, Menglong;Yao, Xuenan;Wang, Haiqi;Xu, Xiaobo;Kong, Junhua;Wang, Yongjian;Fan, Peige;Liang, Zhenchang;Dai, Zhanwu;Liu, Menglong;Yao, Xuenan;Xu, Xiaobo;Kong, Junhua;Wang, Yongjian;Wang, Zixuan;Fan, Peige;Liang, Zhenchang;Dai, Zhanwu;Liu, Menglong;Yao, Xuenan;Wang, Haiqi;Xu, Xiaobo;Kong, Junhua;Wang, Yongjian;Wang, Zixuan;Fan, Peige;Liang, Zhenchang;Dai, Zhanwu;Chen, Weiping;Bai, Huiqing;Wang, Zixuan;Setati, Mathabatha Evodia;Blancquaert, Erna;Crauwels, Sam
关键词：grape; microbiome; terroir;
Vitis vinifera ; volatiles -
Genetic traceability, conservation effectiveness, and selection signatures analysis based on ancestral information: a case study of Beijing-You chicken
作者：Kong, Yuan;Wen, Zilong;Cai, Xinyu;Tan, Lizhi;Liu, Zexuan;Zhao, Yiqiang;Wang, Qiao;Li, Qinghe;Yang, Ning;Wang, Yuzhan;Wang, Yuzhan;Zhao, Yiqiang
关键词：Ancestry analysis; Genetic traceability; Conservation effectiveness; Selection signatures; Beijing-You chicken
-
Identification of regulatory loci and candidate genes related to body weight traits in broilers based on different models
作者：Luo, Na;Cai, Keqi;Cui, Huanxian;Wen, Jie;An, Bingxing;Zhao, Guiping;Luo, Na;Wei, Limin;Zhao, Guiping
关键词：Longitudinal; Genome-wide association studies; Body weight; Chicken 55 K SNP array; Transcriptome
