Cryoprotective Activity of Different Characterized Fractions Isolated from Enzymatic Hydrolysates of Croceine Croaker (Pseudosciaena crocea)
文献类型： 外文期刊
作者： Xu, Zhe 1 ; Cao, Shengao 1 ; Cui, Na 3 ; Zhang, Rui 4 ; Zhao, Shuang 1 ; Zhang, Lijuan 1 ; Guan, Shuang 1 ; Xu, Yikun 1 ; Yan, Xu 1 ; Zhu, Zhixuan 1 ; Tan, Zhijian 2 ; Li, Tingting 1 ;
作者机构： 1.Dalian Minzu Univ, Coll Life Sci, Key Lab Biotechnol & Bioresources Utilizat, Minist Educ, Dalian 116600, Peoples R China
2.Chinese Acad Agr Sci, Inst Bast Fiber Crops & Ctr Southern Econ Crops, Changsha 410205, Peoples R China
3.Liuzhou Inst Technol, Dept Food & Chem Engn, Liuzhou 545616, Peoples R China
4.Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Collaborat Innovat Ctr Seafood Deep Proc, Sch Food Sci & Technol, Dalian 116034, Peoples R China
关键词： cryoprotective; isolation; anti-freezing; trypsin hydrolysates; myofibrillar protein
期刊名称：FOODS （ 影响因子：4.7； 五年影响因子：5.1 ）
ISSN：
年卷期： 2024 年 13 卷 12 期
页码：
收录情况： SCI
摘要： In this study, ultrafiltration fractions (<3 k Da, LMH; >3 k Da, HMH) and solid-phase extraction fractions (hydrophilic hydrolysate, HIH; hydrophobic hydrolysate, HOH) from trypsin hydrolysate purified from croceine croaker (Pseudosciaena crocea) isolate were obtained to investigate the cryoprotective effects of the different fractions, achieved by means of maceration of turbot fish meat after three freeze-thaw cycles. Alterations in the texture, color, moisture loss, myofibrillar protein oxidation stability and conformation, and microstructure of the fish were analyzed after freezing and thawing. The results demonstrate that HIH maximized the retention of fish texture, reduced moisture loss, minimized the oxidation and aggregation of myofibrillar proteins, and stabilized the secondary and tertiary structures of myofibrillar proteins compared to the control group. In conclusion, the HIH component in the trypsin hydrolysates of croceine croaker significantly contributes to minimizing freeze damage in fish meat and acts as an anti-freezing agent with high industrial application potential.
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