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AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta

文献类型： 外文期刊

作者： Song, Zhe 1 ; Li, Yukuo 2 ; Zhan, Xu 2 ; Li, Xiaohan 1 ; Ye, Lingshuai 2 ; Lin, Miaomiao 2 ; Wang, Ran 2 ; Sun, Leiming 2 ; Chen, Jinyong 2 ; Fang, Jinbao 2 ; Wei, Feng 1 ; Qi, Xiujuan 2 ;

作者机构： 1.Zhengzhou Univ, Sch Agr Sci, Zhengzhou 450001, Peoples R China

2.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Zhengzhou 450009, Peoples R China

3.Chinese Acad Agr Sci, Zhongyuan Res Ctr, Xinxiang 453500, Peoples R China

4.Henan Univ Sci & Technol, Coll Hort & Plant Protect, Luoyang 471000, Peoples R China

关键词： Actinidia arguta; Anthocyanin biosynthesis; AaMYB61-like; AabHLH137; Transcription regulation

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundRed Actinidia arguta has recently become highly popular because of its red appearance resulting from anthocyanin accumulation, and has gradually become an important breeding direction. However, regulators involved in anthocyanin biosynthesis have not been fully characterized in A. arguta.ResultsHere, we demonstrated that a key R2R3-MYB transcription factor, AaMYB61-like, plays a crucial role in A. arguta anthocyanin biosynthesis. The RT-qPCR results revealed that transient overexpression of AaMYB61-like in A. arguta fruit at 90-100 DAFB significantly promoted anthocyanin biosynthesis, as did the gene expression levels of AaCHS, AaCHI, AaF3H, AaLDOX, and AaF3GT, whereas the result of VIGS revealed the opposite results in A. arguta fruit at 105-115 DAFB. A transcriptional activation assay indicated that AaMYB61-like exhibited transcriptional activation activity. Y1H and LUC assays revealed that AaMYB61-like activates the promoters of AaCHS, AaLDOX, and AaF3GT. In addition, AabHLH137 was found to be related to fruit color from the transcriptome data. We demonstrated that AaMYB61-like promotes anthocyanin biosynthesis by interacting with AabHLH137 via Y2H, BiFC, and Agrobacterium-mediated co-transformation.ConclusionsOur study not only reveals the functions of AaMYB61-like and AabHLH137 in anthocyanin regulation, but also broadly enriches color regulation theory, establishing a foundation for clarifying the molecular mechanism of fruit coloration in kiwifruit.

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