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Dimethyl carbonate replaces xylene in paraffin section as a biosafe and effective transparent reagent

文献类型： 外文期刊

作者： Chen, Xiaoxia 1 ; Zou, Linen 2 ; Tian, Dongdong 3 ; Sun, Yanguo 3 ; Jin, Xiaoshan 3 ; Xiao, Lixia 3 ; Xue, Luyao 3 ; Song, Wenting 3 ; Wang, Ran 1 ; Wu, Chongming 2 ; Wang, Dawei 3 ;

作者机构： 1.Zhengzhou Univ, Sch Agr Sci, Natl Key Lab Cotton Biobreeding & Integrated Utili, Zhengzhou Res Base, Zhengzhou 450052, Henan, Peoples R China

2.Tianjin Univ Tradit Chinese Med, Sch Chinese Mat Med, Tianjin 301617, Peoples R China

3.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Tobacco Biol & Proc, Qingdao, Peoples R China

5.Shandong Peanut Res Inst, Qingdao, Shandong, Peoples R China

6.Chinese Acad Agr Sci, Grad Sch, Beijing, Peoples R China

7.Henan Agr Univ, Coll Life Sci, Zhengzhou, Peoples R China

关键词： Biosafe; dimethyl carbonate; paraffin section; toxicology; transparent agent; xylene

期刊名称：BIOTECHNIC & HISTOCHEMISTRY （ 影响因子：1.4； 五年影响因子：1.6 ）

ISSN： 1052-0295

年卷期： 2025 年 100 卷 4 期

页码：

收录情况： SCI

摘要： Paraffin section is an important technique in histology research, and transparency is the key step connecting the procedures of dehydration and wax immersion in paraffin sectioning technology. However, the commonly used transparent reagent xylene is toxic to the human body and the environment and included in the list of carcinogens. Few biosafe and environmentally friendly transparent reagents have been widely used to replace xylene. In this study, we found a green transparent reagent, dimethyl carbonate (DMC). Physical and chemical analysis showed that DMC was nontoxic and biodegradable. The feasibility analysis of DMC as a substitute for xylene showed that DMC improved the quality of wax band, staining quality, cell boundary, cell morphology, and section integrity in the sections of plant and animal tissues. The toxicological analysis showed that DMC had no significant effect on the key organs of mice, did not induce obvious inflammation in vivo, and showed higher biosafety than xylene. DMC can be used as a transparent reagent to replace xylene in paraffin section, based on its better physical properties, better paraffin sectioning effect, and higher biosafety. DMC is expected to be widely used in the technical fields of tissue section through further optimization and improvement of the method.

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