文献类型： 外文期刊

作者： Si, Jingfang ¹ ; Dai, Dongmei ¹ ; Gorkhali, Neena Amatya ² ; Wang, Mingshan ³ ; Wang, Sheng ³ ; Sapkota, Saroj ² ; Kadel, Ram Chandra ⁴ ; Sadaula, Amir ⁵ ; Dhakal, Aashish ¹ ; Faruque, Md Omar ⁶ ; Omar, Abdullah Ibne ¹ ; Sari, Eka Meutia ⁷ ; Ashari, Hidayat ⁷ ; Dagong, Muhammad Ihsan Andi ⁸ ; Yindee, Marnoch ⁹ ; Rushdi, Hossam E. ¹⁰ ; Elregalaty, Hussein ¹¹ ; Amin, Ahmed ¹⁰ ; Radwan, Mohamed A. ¹⁰ ; Pham, Lan Doan ¹² ; Hulugalla, W. M. M. P. ¹³ ; Silva, G. L. L. Pradeepa ¹³ ; Zheng, Wei ¹⁴ ; Mansoor, Shahid ¹⁵ ; Ali, Muhammad Basil ¹⁵ ; Vahidi, Farhad ¹⁷ ; Al-Bayatti, Sahar Ahmed ¹⁸ ; Pauciullo, Alfredo ¹⁹ ; Lenstra, Johannes A. ²⁰ ; Barker, J. Stuart F. ²¹ ; Fang, Lingzhao ²² ; Wu, Dong-Dong ³ ; Han, Jianlin ²³ ; Zhang, Yi ¹ ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Biotech Breeding, Minist Agr & Rural Affairs,Natl Engn Lab Anim Bree, Beijing 100193, Peoples R China

2.Natl Anim Sci Res Inst, Natl Anim Breeding & Genet Res Ctr, Nepal Agr Res Council, Khumaltar, Lalitpur, Nepal

3.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Yunnan Lab Mol Biol Domest Anim, Kunming 650023, Peoples R China

4.Govt Gandaki Prov, Minist Ind Tourism Forests & Environm, Pokhara, Nepal

5.Biodivers Conservat Ctr, Natl Trust Nat Conservat, Chitwan, Nepal

6.Bangladesh Agr Univ, Dept Anim Breeding & Genet, Mymensingh 2202, Bangladesh

7.Syiah Kuala Univ, Fac Agr, Dept Anim Sci, Darussalam Banda Aceh 23111, Indonesia

8.Hasanuddin Univ, Anim Sci Fac, Makassar, Indonesia

9.Walailak Univ, Akkhraratchakumari Vet Coll, Thaiburi 222, Nakhon Si Thamm, Thailand

10.Cairo Univ, Anim Prod Dept, Fac Agr, Giza 12613, Egypt

11.Agr Res Ctr, Anim Prod Res Inst, Dokki, Giza, Egypt

12.Natl Inst Anim Sci, Key Lab Anim Cell Technol, Hanoi 100000, Vietnam

13.Univ Peradeniya, Fac Agr, Dept Anim Sci, Peradeniya, Sri Lanka

14.Chinese Acad Agr Sci, Guangxi Buffalo Res Inst, Guangxi Key Lab Buffalo Genet Reprod & Breeding, Nanning 530001, Peoples R China

15.Natl Inst Biotechnol & Genet Engn NIBGE, Faisalabad, Pakistan

16.Univ Karachi, Jamil ur Rehman Ctr Genome Res, Int Ctr Chem & Biol Sci, Karachi, Pakistan

17.Agr Biotechnol Res Inst Iran North Branch ABRII, Dept Genom, Rasht, Iran

18.Al Farabi Univ Coll, Med Lab Tech Dept, Baghdad, Iraq

19.Univ Turin, Dept Agr Forest & Food Sci, I-10095 Grugliasco, TO, Italy

20.Univ Utrecht, Fac Vet Med, Yalelaan 104, NL-3584 CM Utrecht, Netherlands

21.Univ New England, Sch Environm & Rural Sci, Armidale, NSW 2351, Australia

22.Aarhus Univ, Ctr Quantitat Genet & Genom QGG, DK-8000 Aarhus, Denmark

23.Yazhouwan Natl Lab, Sanya 572024, Peoples R China

关键词： buffalo; CSN2; MC1R; population genomics; selection; single-cell RNA-seq

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

ISSN：

年卷期： 2025 年 12 卷 4 期

页码：

收录情况： SCI

摘要： To identify the genetic determinants of domestication and productivity of Asian water buffaloes (Bubalus bubalis), 470 genomes of domesticated river and swamp buffaloes along with their putative ancestors, the wild water buffaloes (Bubalus arnee) are sequenced and integrated. The swamp buffaloes inherit the morphology of the wild buffaloes. In contrast, most river buffaloes are unique in their morphology, but their genomes cluster with the wild buffaloes. The levels of genomic diversity in Italian river and Indonesian swamp buffaloes decrease at opposite extremes of their distribution range. Purifying selection prevented the accumulation of harmful loss-of-function variants in the Indonesian buffaloes. Genes that evolved rapidly (e.g., GKAP1) following differential selections in the river and swamp buffaloes are involved in their reproduction. Genes related to milk production (e.g., CSN2) and coat color (e.g., MC1R) underwent strong selections in the dairy river buffaloes via soft and hard selective sweeps, respectively. The selective sweeps and single-cell RNA-seq data revealed the luminal cells as the key cell type in response to artificial selection for milk production of the dairy buffaloes. These findings show how artificial selection has been driving the evolutionary divergence and genetic differentiation in morphology and productivity of Asian water buffaloes.