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Isolation methods, proliferation, and adipogenic differentiation of adipose-derived stem cells from different fat depots in bovines

文献类型： 外文期刊

作者： Zhu, Ruirui 1 ; Feng, Ye 1 ; Li, Ruirui 1 ; Wei, Kelong 2 ; Ma, Yun 3 ; Liu, Qingyou 4 ; Shi, Deshun 1 ; Huang, Jieping 1 ;

作者机构： 1.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agro Bi, Guangxi Key Lab Anim Breeding Dis Control & Preven, Nanning 530005, Peoples R China

2.Chinese Acad Agr Sci, Buffalo Res Inst, Nanning 530001, Peoples R China

3.Ningxia Univ, Sch Agr, Ningxia 750021, Peoples R China

4.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise B, Foshan 528225, Peoples R China

关键词： Bovine; Adipose-derived stem cells; Isolation; Proliferation; Adipogenic differentiation

期刊名称：MOLECULAR AND CELLULAR BIOCHEMISTRY （ 影响因子：4.3； 五年影响因子：3.6 ）

ISSN： 0300-8177

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The adipose-derived stem cells (ASCs) are a valuable resource for regenerative medicine and essential materials for research in fat deposition. However, the isolation procedure of ASCs has not been standardized and needs to be harmonized; differences in proliferation and adipogenic differentiation of ASCs obtained from different fat depots have not been well characterized. In the present study, we compared the efficiency of ASCs isolation by enzymatic treatment and explant culture methods and the proliferation ability and adipogenic differentiation potential of ASCs isolated from subcutaneous and visceral fat depots. The explant culture method was simple and with no need for expensive enzymes while the enzymatic treatment method was complex, time consuming and costly. By the explant culture method, a larger number of ASCs were isolated from subcutaneous and visceral fat depots. By contrast, fewer ASCs were obtained by the enzymatic treatment method, especially from visceral adipose. ASCs isolated by the explant culture method performed well in cell proliferation and adipogenic differentiation, though they were slightly lower than those by the enzymatic treatment method. ASCs isolated from visceral depot demonstrated higher proliferation ability and adipogenic differentiation potential. In total, the explant culture method is simpler, more efficient, and lower cost than the enzymatic treatment method for ASCs isolation; compared with visceral adipose, subcutaneous adipose is easier to isolate ASCs; however, the visceral ASCs are superior to subcutaneous ASCs in proliferation and adipogenic differentiation.

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