Supplementation with L-kynurenine during in vitro maturation improves bovine oocytes developmental competence through its antioxidative action
文献类型： 外文期刊
作者： Lu, Canqiang 1 ; Feng, Yun 2 ; Tang, Zhenhua 1 ; Jia, Ruru 1 ; Xia, Fan 1 ; Yu, Tingzi 1 ; Wang, Heng 1 ; Mo, Hongfang 1 ; Shi, Deshun 1 ; Lu, Fenghua 1 ;
作者机构： 1.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangxi Key Lab Anim Breeding Dis Control & Preven, Nanning 530005, Peoples R China
2.Maternal & Child Hlth Hosp, Birth Defects Prevent & Control Inst, Dept Genet & Metab Cent Lab, Nanning 530003, Guangxi Zhuang, Peoples R China
3.Chinese Acad Agr Sci, Guangxi Zhuang Nationality Autonomous Reg Buffalo, Minist Agr, Nanning 530001, Peoples R China
关键词： Bovine; Oocytes; In vitro maturation; L-kynurenine; Antioxidant
期刊名称：THERIOGENOLOGY （ 影响因子：2.5； 五年影响因子：2.7 ）
ISSN： 0093-691X
年卷期： 2025 年 233 卷
页码：
收录情况： SCI
摘要： Oxidative stress impairs the developmental potential of oocytes during in vitro maturation (IVM). L-kynurenine (L-KYN), an endogenous metabolite, exhibits antioxidant, anti-inflammatory and neuroprotective effects. This work aimed to evaluate the potential effects of L-KYN on bovine oocyte IVM and its mechanisms. Different concentrations of L-KYN (0, 10, 50, 100, and 200 mu mol/L) were supplemented to bovine oocyte IVM medium. Results shown a notable enhancement in the oocyte maturation rate and the subsequent zygote cleavage and blastocyst formation rate when the L-KYN concentration reached 100 mu mol/L. Further analysis revealed that this treatment effectively down-regulated expression levels of genes to cumulus cell apoptosis (Bax and Caspase3), up-regulated expression levels of genes to cumulus cell expansion (HAS2, PTX3, and PTGS2) and oocyte antioxidant (GPX4 and CAT). L-KYN supplementation also effectively increased glutathione (GSH) levels, promoted oocytes mitochondrial activity, enhanced DNA repairability, deceased reactive oxygen species (ROS) levels, and reduced apoptosis. In conclusion, adding 100 mu mol/L of L-KYN to IVM medium could enhance bovine oocyte quality and the subsequent embryonic development. L-KYN could be a potential antioxidant supplement for bovine oocyte maturation in vitro.
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