Functional characterization of prenyltransferases involved in de novo synthesis of isoprenoids in the leaf beetle Monolepta hieroglyphica
文献类型： 外文期刊
作者： Song, Xuan 1 ; Liu, Chang 3 ; Yi, Chao-qun 1 ; Tang, Zi-yi 1 ; Dhiloo, Khalid Hussain 1 ; Zhang, Tian-tao 1 ; Liu, Wen-tao 1 ; Zhang, Yong-jun 1 ;
作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China
2.Chinese Acad Agr Sci, Zhongyuan Res Ctr, Xinxiang 453500, Peoples R China
3.Ningxia Acad Agr & Forestry Sci, Inst Plant Protect, Yinchuan 750002, Peoples R China
4.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
5.Sindh Agr Univ, Fac Crop Protect, Dept Entomol, Tandojam 70060, Pakistan
6.Agr Univ Hebei, Coll Plant Protect, Baoding 071000, Peoples R China
关键词： Farnesyl diphosphate synthase; Protein prenyltransferases; Enzyme catalysis; Molecular docking and kinetic analysis; Synthesis and transmission; Leaf beetle
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2024 年 280 卷
页码：
收录情况： SCI
摘要： Prenyltransferases play a pivotal role in the isoprenoid biosynthesis and transfer in insects. In the current study, two classes of prenyltransferases (MhieFPPS1 and MhieFPPS2, MhiePFT-beta and MhiePF/GGT-alpha) were identified in the leaf beetle, Monolepta hieroglyphica. Phylogenetic analysis revealed that MhieFPPS1, MhieFPPS2, MhiePFT-beta and MhiePF/GGT-alpha were clustered in one clade with homologous in insects. Moreover, MhieFPPS2 lacked one aspartate-rich motif SARM. Molecular docking and kinetic analysis indicated that the (E)-GPP displayed higher affinity with MhieFPPS1 compared to DMAPP within the binding pocket containing metal binding sites (MG). The other class of prenyltransferases (MhiePFT-beta and MhiePF/GGT-alpha) lack the aspartate-rich motif. Docking results indicated that binding site of MhiePFT-beta involved divalent metal ions (Zn) and bound farnesyl or geranylgeranyl. In vitro, only recombiant MhieFPPS1 could catalyze the formation of (E)-farnesol against different combination of substrates, including IPP/DMAPP and IPP/(E)-GPP, highlighting the importance of SARM for enzyme activities. Kinetic analysis further indicated that MhiePFT-beta operated via Zn2+-dependent substrate binding, while MhiePF/GGT-alpha stabilized the beta-subunit during catalytic reaction. These findings contribute to a valuable insight in to understanding of the mechanisms involved in the biosynthesis and delivery of isoprenoid products in beetles.
- 相关文献
作者其他论文 更多>>
-
Multi-nutrient fertilization-based analysis of fruit quality and mineral element composition during fruit development in Merlot wine grapevines
作者：Wang, Xiaolong;Ji, Xiaohao;Shi, Xiangbin;Liu, Chang;Wang, Zhiqiang;Liu, Fengzhi;Wang, Haibo;Wang, Xiaolong;Ji, Xiaohao;Shi, Xiangbin;Liu, Chang;Wang, Zhiqiang;Liu, Fengzhi;Wang, Haibo;Shao, Xuedong;Zhang, Zhengwen;Zhong, Xiaomin
关键词：fruit quality; mineral element; optimum range; Merlot
-
Advances and Future Perspectives of HER2 Mutations in Non-Small Lung Cancer (NSCLC), Especially in China
作者：Liu, Chang;Li, Tao;Lv, Yahui;Zhou, Xin;Yang, Fan;Dong, Yi;Li, Ruixin;Zhang, Fan;Hu, Yi;Liu, Chang;Li, Tao;Lv, Yahui;Zhou, Xin;Yang, Fan;Dong, Yi;Li, Ruixin;Zhang, Fan;Hu, Yi;Liu, Chang;Liu, Chang;Li, Tao;Cui, Xiaoran;Lv, Yahui;Liu, Min;Zhou, Xin;Yang, Fan;Dong, Yi;Li, Ruixin;Zhang, Fan;Zhang, Tong;Hu, Yi;Li, Tao;Zhang, Tong;Li, Tao;Cui, Xiaoran;Liu, Min
关键词：HER2; Non-small lung cancer (NSCLC); China
-
Juvenile Hormone and Ecdysteroids Facilitate the Adult Reproduction Through the Methoprene-Tolerant Gene and Ecdysone Receptor Gene in the Female Spodoptera frugiperda
作者：Zhang, Yan;Ding, Kui-Ting;Yang, Nian-Wan;Lv, Zhi-Chuang;Wang, Zhen-Ying;Zhang, Yong-Jun;Liu, Wan-Xue;Guo, Jian-Yang;Zhang, Yan
关键词：
Spodoptera frugiperda ; hormone; reproduction;Met ;EcR ; RNA interference -
Mechanism of action and future perspectives of ADCs in combination with immune checkpoint inhibitors for solid tumors
作者：Lv, Yahui;Li, Tao;Liu, Chang;Wang, An;Wang, Ting;Zhou, Xin;Li, Ruixin;Zhang, Fan;Hu, Yi;Liu, Zhefeng;Lv, Yahui;Lv, Yahui;Cui, Xiaoran;Li, Tao;Liu, Chang;Wang, An;Wang, Ting;Zhou, Xin;Li, Ruixin;Zhang, Fan;Hu, Yi;Zhang, Tong;Liu, Zhefeng;Cui, Xiaoran;Li, Tao;Zhang, Tong;Li, Tao;Liu, Zhefeng;Liu, Chang;Wang, Ting
关键词：Antibody-drug conjugates; Immunotherapy; Immune checkpoint inhibitors; Solid tumor
-
Canopy nitrogen and water addition affect fine-root survival strategies and carbon allocation in a warm-temperate forest in China
作者：Li, Wen;Su, Yanzhu;Qin, Yiwen;Li, Fuwei;Zhou, Tenglong;He, Yaqi;Mao, Jianing;Li, Mengke;Liu, Chang;Zhu, Dandan;Shen, Chunyu;Ma, Lei;Fu, Shenglei;Li, Wen;Su, Yanzhu;Qin, Yiwen;Li, Fuwei;Zhou, Tenglong;He, Yaqi;Mao, Jianing;Li, Mengke;Liu, Chang;Zhu, Dandan;Shen, Chunyu;Ma, Lei;Fu, Shenglei;Li, Wen;Su, Yanzhu;Qin, Yiwen;Li, Fuwei;Zhou, Tenglong;He, Yaqi;Mao, Jianing;Li, Mengke;Liu, Chang;Zhu, Dandan;Shen, Chunyu;Ma, Lei;Fu, Shenglei;Li, Wen;Su, Yanzhu;Qin, Yiwen;Li, Fuwei;Zhou, Tenglong;He, Yaqi;Mao, Jianing;Li, Mengke;Liu, Chang;Zhu, Dandan;Shen, Chunyu;Ma, Lei;Fu, Shenglei;Shi, Yifei
关键词：Canopy nitrogen addition; Canopy water addition; Carbon allocation; Functional diversity; Fine roots biomass; Fine roots survival strategy
-
Molecular evidence for the role of the ovipositor of the fall armyworm: Where to lay or not to lay?
作者：Xiao, Yong;Lei, Chun-Mei;Yin, Fei;Peng, Zheng-Ke;Li, Zhen-Yu;Lei, Chun-Mei;Zhang, Yong-Jun;Zalucki, Myron P.;Zalucki, Myron P.
关键词：odorant binding proteins; oviposition behavior; RNA interference; site-directed mutant;
Spodoptera frugiperda -
The potential of biochar to mitigate soil acidification: a global meta-analysis
作者：Zhang, Nanhai;Xing, Jiamin;Wei, Lianjun;Liu, Chang;Zhao, Wenjing;Liu, Zihan;Wang, Yuhao;Ren, Xiaolong;Jia, Zhikuan;Wei, Ting;Zhang, Peng;Zhang, Nanhai;Xing, Jiamin;Wei, Lianjun;Liu, Chang;Zhao, Wenjing;Liu, Zihan;Wang, Yuhao;Ren, Xiaolong;Jia, Zhikuan;Wei, Ting;Zhang, Peng;Zhang, Nanhai;Xing, Jiamin;Wei, Lianjun;Liu, Chang;Wang, Yuhao;Zhang, Peng;Liu, Enke;Siddique, Kadambot H. M.
关键词：Soil acidification; Biochar; Soil pH; Croplands; Amendment
