Beyond species and spatial boundaries: Enabling long-distance gene silencing in plants via guanidinium-siRNA nanoparticles
文献类型： 外文期刊
作者： Lin, Shujin 1 ; Zhang, Qian 2 ; Bai, Shiyan 4 ; Yang, Liwen 2 ; Qin, Guannan 5 ; Wang, Liyuan 4 ; Wang, Wenbin 4 ; Cheng, Cui 4 ; Zhang, Da 4 ; Lu, Chunhua 4 ; Yuan, Jifeng 1 ; Li, Jingying 4 ; Yang, Huanghao 6 ; Gu, Xiaofeng 2 ; Han, Xiao 4 ;
作者机构： 1.Xiamen Univ, Fac Med & Life Sci, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen, Fujian, Peoples R China
2.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing, Peoples R China
3.China Acad Chinese Med Sci, Inst Chinese Mat Med, Artemisinin Res Ctr, Beijing, Peoples R China
4.Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou, Peoples R China
5.Fujian Agr & Forestry Univ, Coll Agr, Key Lab Biol Breeding Fujian & Taiwan Crops, Minist Agr & Rural Affairs, Fuzhou, Peoples R China
6.Fuzhou Univ, Coll Chem, Fuzhou, Peoples R China
关键词： RNA interference; nanoparticle; systematically deliver; thiolated; long-distance movement
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）
ISSN： 1467-7644
年卷期： 2025 年 23 卷 4 期
页码：
收录情况： SCI
摘要： RNA interference (RNAi) has been widely used in agriculture. However, it is well accepted that common methods of plant RNAi are species-dependent and lack systematic efficiency. This study designed a thiolated siRNA nanoparticle, guanidinium (Gu+)-containing disulfide assembled siRNA (Gu+-siRNA), demonstrating remarkable species independence and efficient systemic gene silencing across different plant species. Our results indicate that this approach effectively utilizes the plant vascular system to deliver siRNA, enabling long-distance gene silencing across both monocot and dicot plants, such as rice and Arabidopsis. By applying this method, we successfully targeted and silenced key genes like STM, WER, MYB23, GD1, EIL1, and EIL2, which regulate plant development and enhance salt tolerance. This delivery system significantly expands the application of RNAi technology across different plants, serving as a valuable tool for advancing agricultural biotechnology, enhancing crop resistance, and improving agricultural productivity, while aligning with global goals for sustainable food production and crop improvement.
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