您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Adenovirus-vectored PDCoV vaccines induce potent humoral and cellular immune responses in mice

文献类型： 外文期刊

作者： Miao, Xin 1 ; Zhang, Liping 1 ; Zhou, Peng 1 ; Yu, Ruiming 1 ; Zhang, Zhongwang 1 ; Wang, Cancan 1 ; Guo, Huichen 1 ; Wang, Yonglu 1 ; Pan, Li 1 ; Liu, Xinsheng 1 ;

作者机构： 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Key Lab Anim Virol,Minist Agr, Lanzhou, Peoples R China

2.Gansu Prov Res Ctr Basic Disciplines Pathogen Biol, Lanzhou, Peoples R China

3.Natl Ctr Technol Innovat Pigs, Chongqing, Peoples R China

4.Xujiaping 1, Lanzhou 730046, Gansu, Peoples R China

关键词： Ad5-vector vaccine; PDCoV; Spike glycoprotein; Oral; Immunogenicity

期刊名称：VACCINE （ 影响因子：5.5； 五年影响因子：4.3 ）

ISSN： 0264-410X

年卷期： 2023 年 41 卷 45 期

页码：

收录情况： SCI

摘要： Porcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus that causes severe watery diarrhea, vomiting, dehydration and high mortality in piglets, resulting in significant economic losses by the global pig industry. Recently, PDCoV has also shown the potential for cross-species transmission. However, there are currently few vaccine studies and no commercially available vaccines for PDCoV. Hence, here, two novel human adenovirus 5 (Ad5)-vectored vaccines expressing codon-optimized forms of the PDCoV spike (S) glycoprotein (Ad-PD-tPA-Sopt) and S1 glycoprotein (Ad-PD-oriSIP-S1opt) were constructed, and their effects were evaluated via intramuscular (IM) injection in BALB/c mice with different doses and times. Both vaccines elicited robust humoral and cellular immune responses; moreover, Ad-PD-tPA-Sopt-vaccinated mice after two IM injections with 108 infectious units (IFU)/mouse had significantly higher anti-PDCoV-specific neutralizing antibody titers. In contrast, the mice immunized with Ad-PD-tPA-Sopt via oral gavage (OG) did not generate robust systemic and mucosal immunity. Thus, IM Ad-PD-tPA-Sopt administration is a promising strategy against PDCoV and provides useful information for future animal vaccine development.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台