文献类型： 外文期刊

作者： Sun, Jilin ¹ ; Cui, Xiaokuo ² ; Zhang, Jiaqi ¹ ; Meng, Fansen ¹ ; Li, Jiangong ¹ ; Zhou, Shenghui ³ ; Wu, Changai ² ; Zhou, Peng ³ ; Sun, Silong ¹ ; Han, Long ⁴ ;

作者机构： 1.Shandong Agr Univ, Coll Agron, Natl Key Lab Wheat Improvement, Tai An 271018, Shandong, Peoples R China

2.Shandong Agr Univ, Coll Life Sci, Shandong Engn Res Ctr Plant Microbial Restorat Sal, Natl Key Lab Wheat Improvement, Tai An 271018, Peoples R China

3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Breeding, Beijing 100081, Peoples R China

4.Taian Acad Agr Sci, Tai An 271000, Shandong, Peoples R China

关键词： TeNACs; Th. elongatum; Expression pattern; Salt stress

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundThe NAC (NAM, ATAF1/2, and CUC2) gene family plays a critical role in regulating plant growth, developmental processes, and stress response mechanisms. While NAC genes have been systematically characterized in multiple plant species, this study focused on genome-wide identification of NAC family members in Thinopyrum elongatum (designated as TeNACs) through integrated bioinformatics approaches. Comprehensive analyses were conducted to determine the physicochemical characteristics, conserved motifs, gene structure, phylogenetic relationships, chromosomal collinearity, and expression profiles of the identified TeNACs. This multi-dimensional characterization provides fundamental insights into the structural and functional diversity of NAC transcription factors in Th. elongatum.ResultsA total of 116 NAC transcription factors were systematically identified in Th. elongatum, distributed across all seven chromosomes. Comprehensive physicochemical characterization revealed substantial variation among TeNAC proteins: amino acid length (162-718 aa), molecular weight (18.33-78.25 kDa), isoelectric point (6.5-7.5), instability index (30.34-63.32), and aliphatic index (50.92-80.68). Hydropathicity analysis confirmed the hydrophilic nature of all TeNACs, with grand average values consistently below zero. Conserved motif profiling demonstrated a highly conserved architecture in TeNACs, featuring ordered arrangements of motifs 3, 4, 1, 5, 6, 2, and 7. Phylogenetic reconstruction classified TeNACs into 14 distinct clades through comparative analysis with Arabidopsis thaliana NAC genes, notably lacking ANAC001 and OsNAC8 homologs. Comparative genomic analysis identified significant syntenic conservation between TeNACs and wheat NAC genes. Protein interaction network prediction indicated intricate functional associations among TeNAC proteins. Computational predictions coupled with experimental validation of TeNAC021 confirmed exclusive nuclear localization for all family members. Differential expression analysis across a salt gradient (0-300 mM) identified 14 TeNACs with progressive up-regulation and 5 showing consistent down-regulation. RT-qPCR confirmed salt-responsive expression patterns for eight TeNACs demonstrating marked transcriptional changes.ConclusionsThis systematic investigation establishes a robust theoretical framework for subsequent structural and functional characterization of TeNACs in Th. elongatum.