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Self-Assembling Nanovaccine Fused with Flagellin Enhances Protective Effect against Foot-and-Mouth Disease Virus

文献类型： 外文期刊

作者： Pei, Chenchen 1 ; Dong, Hu 1 ; Teng, Zhidong 1 ; Wei, Sumin 1 ; Zhang, Yun 1 ; Yin, Shuanghui 1 ; Tang, Jianli 1 ; Sun, Shiqi 1 ; Guo, Huichen 1 ;

作者机构： 1.Lanzhou Univ, Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Coll Vet Med,State Key Lab Anim Dis Control & Prev, Lanzhou 730046, Peoples R China

2.Gansu Prov Res Ctr Basic Disciplines Pathogen Biol, Lanzhou 730046, Peoples R China

3.Yangtze Univ, Coll Anim Sci, Jingzhou 434023, Peoples R China

关键词： foot-and-mouth disease; flagellin; self-assembling; nanoparticles; nanovaccine

期刊名称：VACCINES （ 影响因子：7.8； 五年影响因子：7.4 ）

ISSN：

年卷期： 2023 年 11 卷 11 期

页码：

收录情况： SCI

摘要： Nanovaccines based on self-assembling nanoparticles (NPs) can show conformational epitopes of antigens and they have high immunogenicity. In addition, flagellin, as a biological immune enhancer, can be fused with an antigen to considerably enhance the immune effect of antigens. In improving the immunogenicity and stability of a foot-and-mouth disease virus (FMDV) antigen, novel FMDV NP antigens were prepared by covalently coupling the VP1 protein and truncated flagellin containing only N-terminus D0 and D1 (N-terminal aa 1-99, nFLiC) with self-assembling NPs (i301). The results showed that the fusion proteins VP1-i301 and VP1-i301-nFLiC can assemble into NPs with high thermal tolerance and stability, obtain high cell uptake efficiency, and upregulate marker molecules and immune-stimulating cytokines in vitro. In addition, compared with monomeric VP1 antigen, high-level cytokines were stimulated with VP1-i301 and VP1-i301-nFLiC nanovaccines in guinea pigs, to provide clinical protection against viral infection comparable to an inactivated vaccine. This study provides new insight for the development of a novel FMD vaccine.

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