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NF-YB1-YC12-bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)

文献类型： 外文期刊

作者： Bello, Babatunde Kazeem 1 ; Hou, Yuxuan 1 ; Zhao, Juan 1 ; Jiao, Guiai 1 ; Wu, Yawen 1 ; Li, Zhiyong 1 ; Wang, Yifeng; 1 ;

作者机构： 1.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou, Zhejiang, Peoples R China

2.Hubei Univ, Coll Life Sci, State Key Lab Biocatalysis & Enzyme Engn,Hubei Ke, Hubei Collaborat Innovat Ctr Green Transformat Bi, Wuhan, Hubei, Peoples R China

关键词： nuclear factor Y; rice (Oryza sativa L; ); grain quality; Wx gene; G-box; heterotrimer complex

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2019 年 17 卷 7 期

页码：

收录情况： SCI

摘要： Identification of seed development regulatory genes is the key for the genetic improvement in rice grain quality. NF-Ys are the important transcription factors, but their roles in rice grain quality control and the underlying molecular mechanism remain largely unknown. Here, we report the functional characterization a rice NF-Y heterotrimer complex NF-YB1-YC12-bHLH144, which is formed by the binding of NF-YB1 to NF-YC12 and then bHLH144 in a sequential order. Knock-out of each of the complex genes resulted in alteration of grain qualities in all the mutants as well as reduced grain size in crnf-yb1 and crnf-yc12. RNA-seq analysis identified 1496 genes that were commonly regulated by NF-YB1 and NF-YC12, including the key granule-bound starch synthase gene Wx. NF-YC12 and bHLH144 maintain NF-YB1 stability from the degradation mediated by ubiquitin/26S proteasome, while NF-YB1 directly binds to the 'G-box' domain of Wx promoter and activates Wx transcription, hence to regulate rice grain quality. Finally, we revealed a novel grain quality regulatory pathway controlled by NF-YB1-YC12-bHLH144 complex, which has great potential for rice genetic improvement.

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