Discovery and biochemical characterization of prenyltransferases in the biosynthetic pathway of hericenones from Hericium erinaceus
文献类型： 外文期刊
作者： Han, Haiyan 1 ; Peng, Shuang 1 ; Yang, Yuxin 1 ; Lin, Chao 3 ; Wang, Pengchao 1 ; Li, Chang 4 ; Zhao, Peipei 5 ; Gong, Wenbing 6 ; Xie, Chunliang 6 ; Xia, Xuekui 5 ; Qi, Jianzhao 3 ; Liu, Chengwei 1 ;
作者机构： 1.Northeast Forestry Univ, Coll Life Sci, Key Lab Enzyme & Enzyme Like Mat Engn Heilongjiang, Harbin 150040, Heilongjiang, Peoples R China
2.Jining Municipal Ctr Dis Control & Prevent, Jining 272000, Peoples R China
3.Northwest A&F Univ, Coll Chem & Pharm, Shaanxi Key Lab Nat Prod & Chem Biol, Yangling 712100, Peoples R China
4.HarBin Med Univ, Coll Pharm, Dept Med Chem & Nat Med Chem, Harbin 150081, Peoples R China
5.Qilu Univ Technol, Shandong Acad Sci, Biol Inst, Jinan 250103, Peoples R China
6.Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Peoples R China
关键词： Mushroom; Hericium erinaceus; Hericenones; Prenyltransferase; Aspergillus oryzae
期刊名称：BIOORGANIC CHEMISTRY （ 影响因子：4.7； 五年影响因子：4.9 ）
ISSN： 0045-2068
年卷期： 2025 年 164 卷
页码：
收录情况： SCI
摘要： Hericenones, neurotrophic meroterpenoids from the medicinal mushroom Hericium erinaceus, exhibit remarkable nerve growth factor-enhancing properties with therapeutic potential for neurodegenerative disorders. Biosynthesis of these compounds requires prenyltransferase (PT)-mediated geranylation of orsellinic acid (OA, 1), though the fungal enzymes catalyzing this pivotal step remain uncharacterised. Through comprehensive genome mining of H. erinaceus, we identified eight putative UbiA-type prenyltransferases (HePT1-8). Heterologous expression in Aspergillus oryzae coupled with substrate-feeding assays revealed HePT8 as a geranyltransferase capable of 1 geranylation, producing cannabigerorcinic acid (2)-the committed precursor of hericenones. Notably, HePT7 demonstrated divergent specificity for dimethylallyl transfer to 4-hydroxybenzoate. Systematic mutagenesis uncovered critical catalytic residues (H40/D96/D100/D211/D218/Y158) coordinating Mg2+- diphosphate interactions and substrate positioning in HePT8. This work not only elucidates the foundational step in hericenone biosynthesis but significantly expands the catalytic repertoire of fungal PTs through the discovery of multifunctional enzymes with substrate plasticity. Our findings provide essential tools for bioengineering novel meroterpenoids and illuminate evolutionary convergence in fungal secondary metabolism.
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