Isolation of salt-tolerant Vibrio alginolyticus X511 for efficient co-production of 2,3-butanediol and alginate lyase from Laminaria japonica
文献类型： 外文期刊
作者： Xu, Chao 1 ; Xiong, Yaru 2 ; Wang, Qiaozhen 5 ; Fang, Fang 1 ; Wang, Jianhui 1 ; Huang, Shushi 5 ; Xu, Jingliang 6 ; Peng, Yuande 3 ; Xie, Chunliang 3 ;
作者机构： 1.Changsha Univ Sci & Technol, Sch Food & Bioengn, Changsha 410005, Peoples R China
2.Hunan Prov Ctr Dis Control & Prevent, Changsha 410005, Peoples R China
3.Inst Bast Fiber Crops, Changsha 410205, Peoples R China
4.Chinese Acad Agr Sci, Ctr Southern Econ Crops, Engn & Technol Ctr Bast Fiber Crops Hunan Prov, Key Lab Biol & Proc Bast Fiber Crops,Minist Agr &, Changsha 410205, Peoples R China
5.Guangxi Key Lab Marine Nat Prod & Combinatorial Bi, Nanning 530007, Peoples R China
6.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China
关键词： Brown algae; Alginate lyase; 3-butanediol
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 288 卷
页码：
收录情况： SCI
摘要： In order to establish an efficient microbial transformation platform based on seaweed feedstocks, experiments were performed to isolate a salt-tolerant strain capable of producing alginate lyase and 2,3-butanediol (2,3-BDO). Its physiological and biochemical characteristics, carbon source utilization, and product synthesis capabilities were investigated, and then the process for co-producing alginate lyase and 2,3-BDO from Laminaria japonica was optimized. Results showed that the isolated strain was identified as Vibrio alginolyticus, which was capable of utilizing multiple carbon sources to produce alginate lyase and 2,3-BDO even in the presence of 5 % NaCl. The highest reducing sugar yield was achieved as the Laminaria japonica pretreated with 1 % (v/v) sulfuric acid at 120 degrees C for 18 min. The enzymatic hydrolysis was boosted by devising a novel tween 80-assisted enzyme complex containing 60 FPU/g of cellulase, 15 U/g of pectinase, 20 U/g of alginate lyase, and 90 mg/g of tween 80. After establishing a semi-simultaneous saccharification and fermentation (S-SSF) strategy, the sugars could be fully utilized, yielding 14.83 g/L 2,3-BDO and 11.02 kU/L alginate lyase, respectively. Mass balance calculations indicating that up to 140 g of 2,3-butanediol and 120 kU of alginate lyase can be obtained from per kg of Laminaria japonica via this integrated approach.
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