Cell-Type Specific miRNA Regulatory Network Responses to ABA Stress Revealed by Time Series Transcriptional Atlases in Arabidopsis
文献类型： 外文期刊
作者： Gao, Zhaoxu 1 ; Su, Yanning 1 ; Jiao, Guanzhong 1 ; Lou, Zhiying 4 ; Chang, Le 1 ; Yu, Renbo 5 ; Xu, Chao 1 ; Han, Xue 4 ; Wang, Zejia 1 ; Li, Jian 4 ; Deng, Xing Wang 1 ; He, Hang 1 ;
作者机构： 1.Peking Univ, Sch Adv Agr Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
2.Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
3.Chinese Acad Agr Sci CAAS, Inst Crop Sci, Beijing 100081, Peoples R China
4.Peking Univ, Inst Adv Agr Sci, Shandong Lab Adv Agr Sci Weifang, Shandong 261325, Peoples R China
5.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Peoples R China
关键词： cell-type specific; crosstalk; dynamic; M-FFLs; miRNAs; network; rapid; scRNA-seq
期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）
ISSN：
年卷期： 2025 年 12 卷 9 期
页码：
收录情况： SCI
摘要： In plants, microRNAs (miRNAs) participate in complex gene regulatory networks together with the transcription factors (TFs) in response to biotic and abiotic stresses. To date, analyses of miRNAs-induced transcriptome remodeling are at the whole plant or tissue levels. Here, Arabidopsis's ABA-induced single-cell RNA-seq (scRNA-seq) is performed at different stages of time points-early, middle, and late. Single-cell level primary miRNAs (pri-miRNAs) atlas supported the rapid, dynamic, and cell-type specific miRNA responses under ABA treatment. MiRNAs respond rapidly and prior to target gene expression dynamics, and these rapid response miRNAs are highly cell-type specific, especially in mesophyll and vascular cells. MiRNA-TF-mRNA regulation modules are identified by identifying miRNA-contained feed-forward loops (M-FFLs) in the regulatory network, and regulatory networks with M-FFLs have higher co-expression and clustering coefficient (CC) values than those without M-FFLs, suggesting the hub role of miRNAs in regulatory networks. The cell-type-specific M-FFLs are regulated by these hub miRNAs rather than TFs through sc-RNA-seq network analysis. MiR858a-FBH3-MYB module inhibited the expression of MYB63 and MYB20, which related to the formation of plant secondary wall and the production of lignin, through M-FFL specifically in vascular. These results can provide prominent insights into miRNAs' dynamic and cell-type-specific roles in plant development and stress responses.
- 相关文献
作者其他论文 更多>>
-
Broad host adaptation of Mythimna separata mediated by gut bacteria and detoxification enzyme gene MsGSTd3
作者：Xu, Chao;Chen, Ruifang;Zhu, Xiangzhen;Wang, Li;Li, Dongyang;Zhang, Kaixin;Gao, Xueke;Elumalai, Punniyakotti;Ji, Jichao;Luo, Junyu;Cui, Jinjie;Zhu, Xiangzhen;Wang, Li;Li, Dongyang;Zhang, Kaixin;Gao, Xueke;Ji, Jichao;Luo, Junyu;Cui, Jinjie;Zhu, Xiangzhen;Wang, Li;Li, Dongyang;Zhang, Kaixin;Gao, Xueke;Ji, Jichao;Luo, Junyu;Cui, Jinjie;Xu, Chao;Xu, Chao;Chen, Ran;Chen, Ruifang
关键词：Oriental armyworm; host plant; life history traits; 16S rRNA sequencing; Enterobacter cloacae; Enterococcus mundtii; RNAi
-
Epigenetics in the modern era of crop improvements
作者：Xue, Yan;Deng, Xing Wang;Cao, Xiaofeng;Deng, Xian;Cao, Xiaofeng;Deng, Xian;Chen, Xiangsong;Yang, Hongchun;Ding, Yong;Dong, Aiwu;Liu, Bing;Ren, Guodong;Zhang, Yijing;Duan, Cheng-Guo;Fang, Xiaofeng;Qi, Yijun;Gong, Lei;Liu, Bao;Xu, Zheng-Yi;Gong, Zhizhong;Gong, Zhizhong;Gu, Xiaofeng;He, Chongsheng;He, Hang;He, Shengbo;He, Xin-Jian;He, Yan;Tian, Zhixi;He, Yuehui;Jia, Guifang;Wang, Yuan;Jiang, Danhua;Song, Xianwei;Jiang, Jianjun;Lai, Jinsheng;Lai, Jinsheng;Lai, Jinsheng;Lai, Jinsheng;Lai, Jinsheng;Lang, Zhaobo;Zhu, Jian-Kang;Li, Chenlong;Li, Qing;Li, Xingwang;Yan, Wenhao;Zhou, Dao-Xiu;Luo, Xiao;Qian, Weiqiang;Song, Qingxin;Wang, Jia-Wei;Xu, Lin;Wu, Liang;Wu, Zhe;Xia, Rui;Xiao, Jun;Zhao, Yusheng;Zhai, Jixian;Zhong, Xuehua;Zhou, Dao-Xiu;Zhou, Ming;Zhou, Yue;Zhu, Bo;Liu, Qikun
关键词：epigenetics; plant development; plant immunity; crop improvement; stress responses
-
Advancements in lignocellulosic biorefining: An integrated approach for achieving complete conversion of unsterilized peanut shells into high-titer ethanol and succinic acid
作者：Xu, Chao;Zhou, Wen;Fang, Fang;Wang, Jianhui;Liu, Jun;Xiong, Yaru;Xu, Chao;Peng, Yuande;Xie, Chunliang;Liu, Dandan;Wu, Yucui;Peng, Yuande;Xie, Chunliang;Liu, Dandan;Wu, Yucui;Peng, Yuande;Xie, Chunliang;Huang, Shushi;Xu, Chao
关键词：Lignocellulosic biomass; Cellulosic ethanol; Co-fermentation
-
Preparation of cellulose-based nanoparticles via electrostatic self-assembly for the pH-responsive delivery of astaxanthin
作者：Gan, Miaoyu;Cai, Luyun;Luan, Qian;Gan, Miaoyu;Cai, Luyun;Luan, Qian;Cao, Ailing;Cai, Luyun;Luan, Qian;Xiang, Xia;Li, Jian
关键词：Astaxanthin; Cellulose nanofibers; Nanoparticles; pH-responsive; Controlled release
-
Enhancing the freeze-thaw stability of maize starch via targeted mutation of both Waxy1 and Sugary2
作者：Ma, Mingzheng;Liu, Peifeng;Zhu, Jinjie;Gao, Zhaoxu;Qi, Xiantao;Xie, Chuanxiao;Liu, Changlin;Xie, Chuanxiao;Liu, Changlin
关键词：Maize; CRISPR/Cas9; freeze-thaw stability; Waxy1; Sugary2
-
Effect of novel radio frequency roasting on color appearance, volatile compounds, fatty acid composition, and storage stability of peanut oil
作者：Peng, Zekang;Xu, Chao;Zhang, An'an;Liu, Yanhong;Zhang, Yue;Ai, Ziping
关键词：Peanut oil; Radio frequency roasting; Physicochemical quality; Flavor; Sensory properties; Maillard reaction; Fourier transform infrared spectroscopy
-
Comparative analysis of extraction technologies for plant extracts and absolutes
作者：Cao, Shoutao;Chen, Mingguang;Xu, Chao;Qiu, Lei;Zhao, Xianyan;Hu, Wenxiao;Liang, Jinchang;Wang, Xiaoqiang
关键词：plant extract; plant absolute; conventional extraction technology; green extraction techniques; green extraction solvent
