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Treatment of lactoferrin and antimicrobial peptide N6 on bacterial enteritis caused by Escherichia coli in mice

文献类型： 外文期刊

作者： Ma, Xuanxuan 1 ; Zhang, Kun 1 ; Yang, Na 1 ; Hao, Ya 1 ; Mao, Ruoyu 1 ; Teng, Da 1 ; Wang, Jianhua 1 ;

作者机构： 1.Chinese Acad Agr Sci, Feed Res Inst, Gene Engn Lab, Innovat Team Antimicrobial Peptides & Alternat Ant, Beijing 100081, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Feed Biotechnol, Beijing 100081, Peoples R China

关键词： ETEC; antimicrobial peptide; bovine lactoferrin; intestinal mucosal immunity; intestinal morphology; intestinal tight junction proteins

期刊名称：BIOCHEMISTRY AND CELL BIOLOGY （ 影响因子：2.1； 五年影响因子：2.3 ）

ISSN： 0829-8211

年卷期： 2025 年 103 卷

页码：

收录情况： SCI

摘要： Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in humans and animals. The study aimed to evaluate the efficacy of bovine lactoferrin (bLf) as an adjuvant combined with AMP (N6) in the treatment of E. coli-induced bacterial enteritis. Firstly, 40 female ICR mice were randomly divided into four groups. The ETEC-A, ETEC-B, and ETEC-C groups were gavaged with 0.2 mL of ETEC K88 at 5 x 10(9), 5 x 10(8), and 5 x 10(7) CFU/mL for three consecutive days, respectively, the CK control group was given PBS. Based on the clinical symptoms and intestinal changes, the optimal model dose of ETEC K88 was determined to be 5 x 108 CFU/mL. Sixty female ICR mice were randomly divided into six groups: CK group (uninfected), NC group (infected and untreated), N6 treatment group (20 mg/kg), bLf treatment group (100 mg/kg), bLf + N6-A treatment group (10 mg/kg N6+100 mg/kg bLf), and bLf + N6-B group (20 mg/kg N6+100 mg/kg bLf). The clinical symptoms, intestinal morphology, inflammatory response and serum metabolites were monitored. The results showed that compared with the NC group, the bLf-N6-A and bLf-N6-B treatment groups had significant reductions in TNF-alpha and IL-6, significant increases in IL-10, and significant reductions in endotoxin and DAO in plasma (p < 0.05). Meanwhile, the bLf-N6-A and bLf-N6-B treatment groups significantly increased the expression of ZO-1, claudin-1 and occludin, increased the height of small intestinal mucosal villi and VH/CD after ETEC K88-induced intestinal injury (p < 0.05). The combination of bLf and N6 relieved enteritis by balancing intestinal mucosal immunity, improving intestinal morphology and barrier function. BLf combined with N6 can be used as an effective therapeutic strategy for the treatment of bacterial enteritis.

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