文献类型： 外文期刊

作者： Zhang, Jie ¹ ; Gu, Riliang ² ; Miao, Xinxin ³ ; Schmidt, Renate H. ⁴ ; Xu, Zhenxiang ² ; Lu, Jiawen ¹ ; Ma, Yuting ¹ ; Yang, Tao ⁵ ; Wang, Pingxi ¹ ; Liu, Yangyang ¹ ; Wang, Xiaoli ¹ ; Du, Xuemei ² ; Zheng, Nannan ² ; Zhen, Sihan ¹ ; Liang, Chengyong ³ ; Xie, Yuxin ¹ ; Wu, Yongrui ⁵ ; Li, Lin ³ ; Reif, Jochen C. ⁴ ; Jiang, Yong ⁴ ; Wang, Jianhua ² ; Fu, Junjie ¹ ; Zhang, Hongwei ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improve, State Key Lab Crop Gene Resources & Breeding, Beijing 100081, Peoples R China

2.China Agr Univ, Coll Agron & Biotechnol, Minist Agr & Rural Affairs, State Key Lab Maize Biobreeding,Beijing Innovat Ct, Beijing 100193, Peoples R China

3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

4.Leibniz Inst Plant Genet & Crop Plant Res IPK, Dept Breeding Res, D-06466 Stadt Seeland, Germany

5.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

6.Sichuan Agr Univ, State Key Lab Crop Gene Explorat & Utilizat Southw, Chengdu 611130, Peoples R China

7.Henan Inst Sci & Technol, Sch Agr, Xinxiang 453003, Peoples R China

8.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China

关键词： hybrid maize; mid-parent heterosis; dominance; heterotic gene; bZIP29

期刊名称：PLANT COMMUNICATIONS （ 影响因子：11.6； 五年影响因子：11.8 ）

ISSN： 2590-3462

年卷期： 2025 年 6 卷 5 期

页码：

收录情况： SCI

摘要： Understanding the role of heterotic genes in contributing to heterosis is essential for advancing hybrid breeding. We analyzed plant height (PH), ear height (EH), and transcriptomic data from a maize hybrid population. Genome-wide association studies (GWASs) revealed that dominance effects of quantitative trait loci (QTLs) play a significant role in hybrid traits and mid-parent heterosis. By integrating GWAS, expression GWAS (eGWAS), and module eGWAS analysis, we prioritized six candidate heterotic genes underlying six QTLs, including one QTL that spans the bZIP29 gene. In the hybrid population, bZIP29 exhibits additive expression and dominance effects for both hybrid traits and mid-parent heterosis, with its favorable allele correlating positively with PH and EH. bZIP29 demonstrates dominance or over-dominance patterns in hybrids derived from crosses between transgenic and wild-type lines, contingent upon its expression. A tsCUT&Tag assay revealed that bZIP29 protein binds directly to a gene regulated by its associated expression QTL (eQTL) and six genes within expression modules governed by its associated module-eQTLs (meQTLs). Regulatory networks involving bZIP29 are more extensive in hybrid subpopulations than in the parental population. This study offers insights into key heterotic genes and networks that underpin the robust growth of hybrid maize.