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PCR-RFLP assay confirms the existence of different mitochondrial lineages of Taenia hydatigena including a possible geographically restricted group

文献类型： 外文期刊

作者： Ohiolei, John Asekhaen 1 ; Yan, Hong-Bin 1 ; Li, Li 1 ; Alvi, Mughees Aizaz 1 ; Muku, Rosline James 1 ; Wu, Yao-Don 1 ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Natl Profess Lab Anim Echinococcosis, Key Lab Vet Parasitol Gansu Prov,Lanzhou Vet Res, Lanzhou 730046, Gansu, Peoples R China

2.Jilin Univ, Coll Vet Med, OIE Collaborating Ctr Foodborne Parasites Asian P, Key Lab Zoonoses Res,Minist Educ,Inst Zoonoses, Changchun, Peoples R China

3.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

关键词： cysticercosis; genetic diversity; haplogroup; PCR-RFLP; phylogeny

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：5.005； 五年影响因子：4.622 ）

ISSN： 1865-1674

年卷期：

页码：

收录情况： SCI

摘要： Taenia hydatigena is a widespread tapeworm of canids (primarily dogs) that causes cysticercosis in ruminants (domestic and wild) and manifests as depression and weakness secondary to various hepatic damages and sometimes mortality in young animals, although, commonly encountered cases are asymptomatic. In most taeniids, genetic polymorphism has been found to impact host preferences, distribution, disease epidemiology and management. Recently, we identified two main mitochondrial lineages of T. hydatigena in China, and here, we examined the mitochondrial nad4-nad5 genes of T. hydatigena from China, Nigeria, Pakistan and Sudan to assess the intraspecies variation of isolates from these countries and also the distribution of the distinct mitochondrial groups. In addition to China, haplogroup B variant was found in Pakistan, while haplogroup A demonstrated a widespread distribution. We then designed a PCR-restriction fragment length polymorphism (PCR-RFLP) assay using Xmil (Acct) and Rsal (Afal) restriction enzymes to differentiate members of both haplogroups. This result provides more molecular evidence supporting the existence of distinct mitochondrial variants of T. hydatigena. The epidemiological significance of these different mitochondrial groups remains to be explored further. The current PCR-RFLP assay offers a useful molecular approach for investigating the genetic population structure of T. hydatigena in enzootic regions and in identifying/discriminating the different mitochondrial groups (haplogroups A and B).

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