Functional identification of Medicago truncatula MtRAV1 in regulating growth and development
文献类型: 外文期刊
作者: Wang, Shumin 1 ; Guo, Tao 3 ; Zhang, Shaolin 2 ; Yang, Hong 2 ; Li, Li 2 ; Yang, Qingchuan 1 ; Quan, Junping 2 ; Long, Ruicai 1 ;
作者机构: 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
2.Nanshan Bot Garden Management Off, Chongqing 400065, Peoples R China
3.Chongqing Landscape & Gardening Res Inst, Chongqing Key Lab Germ plasm Innovat & Utilizat Na, Chongqing 401329, Peoples R China
关键词: RAV transcription factor; growth and development; photosynthesis; plant hormone signal transduction
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:4.4; 五年影响因子:4.8 )
ISSN: 2095-3119
年卷期: 2025 年 24 卷 5 期
页码:
收录情况: SCI
摘要: Related to ABI3 and VP1 (RAV) transcription factors belong to the AP2 and B3 superfamily. RAVs genes have been reported to be involved in plant growth and development regulation. This study screened three RAV genes from Medicago truncatula and named one of them MtRAV1. The MtRAV1 overexpressing plants exhibits traits such as plant dwarfing, delayed flowering, reduced leaf and floral organs, increased branching, and reduced pods and seeds. Gene expression analysis results showed that overexpression of MtRAV1 inhibited the expression of Flowering Locus and Carotenoid Cleavage Dioxygenase 7 (MtCCD7). To further investigate the regulation pathway involved by MtRAV1, RNA-sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analysis were conducted. RNA-seq results indicated that MtRAV1 might affect plant growth and development by regulating some genes in photosynthesis, circadian rhythm and plant hormone signaling pathways, especially the auxin signaling pathway. Conjoint analysis of DAP-seq and RNA-seq revealed that MtRAV1 might inhibit the expression of Ferredoxin (MtFd-l3), Light-harvesting Chlorophyll a/b Binding Protein 1 (MtLhcb-l2) and Small Auxin Up-regulated RNA (MtSAUR-l), which related to photosystem II and auxin signaling pathway. Summarily, MtRAV1 was preliminarily proven to be a key \growth inhibitory factor in M. truncatula.
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